Artikel
The neuroprotective potential of recombinant human granulocyte colony-stimulating factor (G-CSF) after partial lesion on the Vestibulocochlear nerve: An experimental study in a rat model
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Autoren
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Sarah Alkilani
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg, Deutschland
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Simon Müller
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg, Deutschland
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Joachim Oertel
- Universitätsklinikum des Saarlandes, Klinik für Neurochirurgie, Homburg, Deutschland
| Veröffentlicht: | 18. Juni 2018 |
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Gliederung
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Objective: Injuries of the eighth cranial nerve during operation on the cerebellopontine angle (CPA) are common. However, substances that support the nerve’s regeneration are not well established. G-CSF is known to have a neuroprotective effect but literature about its influence on the regeneration of the cochlear nerve is sparse. The aim of this study was to determine the neuroprotective effect of G-CSF after partial cochlear nerve lesion in a rat model.
Methods: Male Sprague Dawley rats (n=35) received a lesion to the right cochlear nerve using a water jet dissector. After operation, the rats received subcutaneous G-CSF- (30µg/kg BW) or NaCl- injections daily for 6 days. Additionally, 14 rats received a preoperative G-CSF/NaCl-injection. To evaluate the influence of G-CSF on the postoperative regeneration of the cochlear nerve, brainstem, auditory evoked potentials (BAEP) were measured pre- and postoperatively, as well as on day 7 and 14 after surgery. After euthanizing at day 7 or 14 histological sections of the cochlear nuclei were prepared.
Results: BAEPs showed that the amplitude of wave II is significantly improved 7 days after surgery. However, there was only a benefit in the amplitude of wave IV in the G-CSF-treated rats. In contrast to the treatment groups, the histological results showed a significantly fewer number of neurons in the control groups. Immunohistochemically, Ki-67 as well as Bcl-2 were overexpressed, whereas Bax was significantly downregulated. Additionally, G-CSF receptor expression was significantly higher in the G-CSF group than in the control group. Furthermore, immunocytochemical results showed that G-CSF reduced the expression of GFAP which reduced glial scar formation and improved axonal growth. There was no difference in the BAEPs 14 days after surgery compared to 7 days after surgery. The preoperative injection of G-CSF had no significant additional benefit on the postoperative nerve regeneration.
Conclusion: The perioperative use of G-CSF in case of iatrogenic cochlear nerve injuries seems promising for an improved outcome.Consequently, clinical research to prove the neuroprotective effect of G-CSF is indicating.
