Artikel
Pain inhibition through transplantation of neuronal progenitors into the injured spinal cord in rats
Suche in Medline nach
Autoren
-
Guilherme Lepski
- Universitätsklinikum Tübingen, Klinik für Neurochirurgie, Tübingen, Deutschland; São Paulo University Medical School, Neurology, São Paulo, Brasilien
-
Chary Martin Marquez Batista
- São Paulo University Medical School, Neurology, São Paulo, Brasilien
-
Eric Domingos Mariano
- São Paulo University Medical School, Neurology, São Paulo, Brasilien
-
Fernando Onuchic
- São Paulo University Medical School, Neurology, São Paulo, Brasilien
-
Camila Squarzoni Dale
- São Paulo University, Institute of Biomedical Science, São Paulo, Brasilien
-
Alexandre Fogaça Cristante
- São Paulo University Medical School, Orthopedic and Traumatology, São Paulo, Brasilien
-
José Pinhata Otoch
- São Paulo University Medical School, Surgery, São Paulo, Brasilien
-
Manoel Jacobsen Teixeira
- São Paulo University Medical School, Neurology, São Paulo, Brasilien
-
Matthias Morgalla
- Universitätsklinikum Tübingen, Klinik für Neurochirurgie, Tübingen, Deutschland
-
Marcos Tatagiba
- Universitätsklinikum Tübingen, Klinik für Neurochirurgie, Tübingen, Deutschland
| Veröffentlicht: | 18. Juni 2018 |
|---|
Gliederung
Text
Objective: Neuropathic pain is caused by a CNS lesion. Myelopathic pain is a subtype that very often aggravates the clinical picture in spinal cord injury (SCI) patients. The pain suppression system is composed of distinct types of neurons, especially serotoninergic ones. Here we evaluated the ability of fetal neural stem cells (fNSC) originating from telencephalic vesicle (TV) and ventral medulla (VM) to generate serotoninergic neurons and to relieve chronic pain after SCI.
Methods: Wistar rats were immunosuppressed with cyclosporine and submitted to traumatic SCI using the NYU Impactor (drop height 25mm) at 9-10th thoracic level. After 10 days, the animals received four intra-spinal injections of culture medium (sham) or fNSC extracted from TV (TV group) or VM (VM group) of E/14 embryos. Behavioral assessment was performed over 8 weeks. Thereafter, spinal cords were processed for immunohistochemistry. The amount of serotoninergic and GABAergic neurons derived from implanted cells was determined by stereology.
Results: Thermal pain threshold improved by ~47.5% in TV group in relation to sham (p<0.05) at the 7th and 8th week after transplantation and improved by 45.9% (p<0.05) and 59.3% (p<0.01) in VM group at the 7th and 8th week, respectively, in relation to sham. Mechanical allodynia, assessed by vonFrey filaments, improved by 52.1% in TV group (p<0.05) and by 49.8% in VM group (p<0.01) at the 8th week. None of treated groups showed motor recovery comparing to sham. Stereological analyses showed that 69.4% of TV cells and 72.1% of VM cells differentiated into neurons, moreover, we counted a higher proportion of GABAergic cells in the TV group (TV vs VM, p<0.05) and a higher proportion of serotoninergic cells in the VM group (TV vs VM, p<0.001).
Conclusion: Neuronal precursors from TV and VM generate different proportions of mature phenotypes (GABA and 5HT), both of which are able to reduce pain perception after SCI.
