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68. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC)
7. Joint Meeting mit der Britischen Gesellschaft für Neurochirurgie (SBNS)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

14. - 17. Mai 2017, Magdeburg

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Acute changes in brain metabolism in the early phase following experimental subarachnoid hemorrhage (SAH)

Meeting Abstract

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  • Nadine Lilla - Würzburg, Deutschland
  • Hester Berger - Departments of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology (NTNU), Trondheim, Norway
  • Ursula Sonnewald - Department of Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway
  • Deborah Hill - Department of Circulation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway
  • Marius Wideroe - Department of Circulation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway
  • Ralf-Ingo Ernestus - Neurochirurgische Klinik und Poliklinik, Universitätsklinikum Würzburg, Würzburg, Deutschland
  • Thomas Westermaier - Neurochirurgische Klinik und Poliklinik, Universitätsklinikum Würzburg, Würzburg, Deutschland

Deutsche Gesellschaft für Neurochirurgie. Society of British Neurological Surgeons. 68. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie (DGNC), 7. Joint Meeting mit der Society of British Neurological Surgeons (SBNS). Magdeburg, 14.-17.05.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. DocDI.22.01

doi: 10.3205/17dgnc300, urn:nbn:de:0183-17dgnc3006

Veröffentlicht: 9. Juni 2017

© 2017 Lilla et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.

Gliederung

Text

Objective: Metabolic changes following subarachnoid hemorrhage (SAH) with accumulation of metabolic products such as lactate, pyruvate and glutamate are described, the exact metabolic derangement and pathophysiology behind still not well understood. Especially information on metabolic changes in the early hours following SAH is rare, possibly explaining an onset of secondary brain damage during course of disease. This study was conducted to further investigate acute metabolic changes in the early phase following experimental SAH studied for the first time with in vivo injection of 13C-labeled glucose and acetate combined with ex vivo 13C magnetic resonance spectroscopy (MRS).

Methods: 18 male Sprague Dawley rats (250g-300g) were randomly assigned to one of two groups: 1) SAH induced by the endovascular filament model or 2) sham operated control animals. All animals received in vivo injection of 13C-labeled glucose and acetate before they were euthanized after 3 hours following SAH or sham operation. Ex vivo 13C MRS and HPLC of brain extracts were performed to study acute metabolic changes

Results: Three hours after experimental SAH, glycolysis was reduced in SAH hemispheres and mitochondrial metabolism in neurons was significantly impaired compared to sham operated rats. In detail, GABAergic neurons were not as much affected as Glutamatergic neurons and astrocyte metabolism seems to be more preserved.

Conclusion: For the first time, we could show significant metabolic changes in rat brains already 3 hours after SAH measured by 13C labelled MRS, giving more insight into the pathophysiology and detail of metabolic changes. Therefore, there is a potential treatment option already in the acute phase when the patient is entering the clinic, trying to prevent from secondary brain damage. Further studies will have to investigate the beneficial neuroprotective effect of possible therapeutic drugs.