Artikel
S100B protein reduces delayed neuronal injury in stretch injured mixed glial-neuronal cell cultures
S100B-Protein reduziert die protrahierte neuronale Dysfunktion nach stretch injury von gemischt neuronal-glialen Zellkulturen
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Autoren
Veröffentlicht: | 23. April 2004 |
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Gliederung
Text
Objective
S100B protein has been localized primarily to astrocytes and is increased in astrocytes and in extracellular fluid after in vitro or in vivo brain trauma or ischemia. Experimental evidence has suggested that S100B release following injury may serve in a paracrine function to reduce neuronal injury or promote neuronal recovery. We examined whether S100B alters the response of neuronal plus glial cell cultures to strain (stretch) injury.
Methods
Following injury, astrocyte nuclei normally take up the dye propidium iodide (PrI) at 15 minutes after injury but by 24 and 48 hr the astrocytes repair and regain their capacity to exclude PrI. In an opposite manner, stretch injured neurons do not take up PrI until approximately 24-48 hr after injury. We examined the spontaneous release of S100B, and the effect of S100B (10 or 100 nM) added at different time points on the PrI uptake of astrocytes and neurons for 48hr after stretch injury.
Results
The spontaneous S100B release started immediately after injury and incresed up to 60ng/l at 48hr. In neurons, 10 or 100 nM S100B given at 6hr and at 24 hr after injury reduced PrI uptake at 48 hr by 30% (p < 0.05). S100B given 15 min before or within 5 sec of injury had no consistent effect on astrocyte PrI uptake at 15 min after injury.
Conclusions
These results support a possible protective or recovery-enhancing role for S100B in neurons following injury. Thereby, post -traumatic release of S100B by astrocytes may contribute to endogenous repair mechanisms.