gms | German Medical Science

131. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

25.03. - 28.03.2014, Berlin

Artikel

Artikel empfehlen

Non-invasive screening for acute rejection in murine renal transplantation using MR renography and diffusion weighted MRI

Meeting Abstract

Suche in Medline nach

  • Aivars Kalnins - Klinikum Großhadern, LMU München, Chirurgie, München
  • Michael Thomas - Klinikum Großhadern, LMU München, Chirurgie, München
  • Markus Rentsch - Klinikum Großhadern, LMU München, Chirurgie, München
  • Jose Torrealba - University of Texas, Southwestern Medical Center, Department of Pathology, Dallas
  • Karl-Walter Jauch - Klinikum Großhadern, LMU München, Chirurgie, München
  • Bruno Meiser - Klinikum Großhadern, LMU München, Transplantationszentrum, München
  • Markus Guba - Klinikum Großhadern, LMU München, Chirurgie, München
  • Jens Werner - Klinikum Großhadern, LMU München, Chirurgie, München
  • Mike Notohamiprodjo - Klinikum Großhadern, LMU München, Radiologie, München
  • Joachim Andrassy - Klinikum Großhadern, LMU München, Chirurgie, München

Deutsche Gesellschaft für Chirurgie. 131. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 25.-28.03.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14dgch543

doi: 10.3205/14dgch543, urn:nbn:de:0183-14dgch5433

Veröffentlicht: 21. März 2014

© 2014 Kalnins et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Introduction: Biopsies are often required to detect the underlying condition for deterioration of the transplant function. Here, we tested a novel substance which inhibits the MCP1/CCR2 pathway via oligonucleotides in a murine renal Tx model. The aim was 1.) to detect potential effects of this drug on acute rejection processes and 2.) show that MR-renography in combination with diffusion weighted imaging (DWI) may be a valuable new tool to non-invasively monitor these rejection processes.

Material and methods: Kidneys of Balb/c mice were transplanted onto B6. Mice were either treated with the anti-MCP1-Spiegelmer in monotherapy or in combination with subtherapeutic CsA (10mg/kgBW). For further analysis immunohistochemistry, doppler ultrasound, MR-renography and diffusion-weighted MRI were used.

Results: The number of F4/80+ cells was efficiently suppressed and kidney cortex perfusion measurements improved under combination therapy. The apparent diffusion coefficient (ADC) of native kidneys and syngenic allografts did not show significant differences. Allogenic allografts without treatment showed significantly lower ADC (p<0.001). Under combination therapy the ADC significantly improved (p=0.002).

Conclusion: The novel drug based on oligonucleotide technology inhibiting the MCP1 alleviates acute rejection. Diffusion-weighted MRI in combination with MR-renography may serve as a valuable tool to detect rejection processes.