gms | German Medical Science

130. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

30.04. - 03.05.2013, München

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Enhancement of bone healing by administration of human parathyroid hormone (PTH 1-34) in male rats

Meeting Abstract

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  • Ewa Klara Stürmer - Universität Witten/Herdecke, Institut für Forschung in der Operativen Medizin, Köln
  • Stephan Sehmisch - Universitätsmedizin Göttingen, Klinik für Unfallchirurgie, Plastische und Wiederherstellungschirurgie, Göttingen
  • Mohammad Tezval - Universitätsmedizin Göttingen, Klinik für Unfallchirurgie, Plastische und Wiederherstellungschirurgie, Göttingen
  • Marina Komrakova - Universitätsmedizin Göttingen, Klinik für Unfallchirurgie, Plastische und Wiederherstellungschirurgie, Göttingen
  • Klaus Michael Stürmer - Universitätsmedizin Göttingen, Klinik für Unfallchirurgie, Plastische und Wiederherstellungschirurgie, Göttingen

Deutsche Gesellschaft für Chirurgie. 130. Kongress der Deutschen Gesellschaft für Chirurgie. München, 30.04.-03.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13dgch546

doi: 10.3205/13dgch546, urn:nbn:de:0183-13dgch5462

Veröffentlicht: 26. April 2013

© 2013 Stürmer et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Introduction: Intermittently administrated PTH and teriparatide (PTH 1-34) are currently being used as therapies for osteoporosis. Recent studies have demonstrated promoting effect of PTH on fracture healing in both healthy and estrogen deficient female rats. In present study we investigated for the first time the effect of PTH on bone healing in aged orchiectomized male rats or controls.

Material and methods: Thirty six Sprague-Dawley male rats were orchiectomized at 8-month of age. Twenty four rats were sham operated. After 12 weeks, when orchiectomized (Orx) rats developed osteoporotic changes, a bilateral transverse metaphyseal osteotomy of tibia was performed in all rats. Both tibia were metaphysealy osteotomized. Rats were divided into SHAM-vehicle, SHAM-PTH daily (PTH/d), ORX-vehicle, ORX-PTH daily (PTH/d), ORX-PTH every other day (PTH/2d). Dosage of PTH was 40µg/kgBW. After 5-weeks, tibiae were analyzed by fpQCT, biomechanical, histolomorphometrical and regarding gene expression. Fiber cross-sectional area, capillary density, and muscle enzyme activity were measured in soleus, gastrocnemius, and longissimus muscles.

Results: In sham rats capillary density was increased in limb muscles (MS: 1.3–1.7, MG: 1.2–1.4 capillaries/fiber), and rate of osseous bridging of osteotomy was enhanced (67–100%). In ORX serum creatine kinase was decreased (6670–2847 U/l), and bone genes (IGF-1, OPG, RANKL) were up-regulated. Cortical and callus densities (Ct.BMD and Cl.BMD), callus area were increased (Cl.BMD: sham: 328 to 438 mg/cm3, Orx 327 to 448 mg/cm3; 410 mg/cm3 in Orx-PTH/2d. PTH improved tibia healing in SHAM, which had lower rate of osteotomy bridging and up-regulated some bone genes in the callus of ORX.

Conclusion: Concluding, PTH applied daily appeared to have favorable effects on muscle tissue and bone healing in both hypogonadal and healthy male rats. Administration of PTH with less frequency proved to be of limited success. PTH had no adverse effect on muscle structure, BW, nor weight of visceral adipose tissue and organs. However, there is concern that the changes related to the lack of androgens might blunt the effect of PTH to some extent.