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130. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

30.04. - 03.05.2013, München

Apoptosewege im Fettgewebe

Meeting Abstract

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  • Christian Herold - Sana Klinikum Hameln-Pyrmont, Plastische und Ästhetische Chirurgie, Hameln

Deutsche Gesellschaft für Chirurgie. 130. Kongress der Deutschen Gesellschaft für Chirurgie. München, 30.04.-03.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13dgch394

doi: 10.3205/13dgch394, urn:nbn:de:0183-13dgch3940

Veröffentlicht: 26. April 2013

© 2013 Herold.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

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Einleitung: Fat cells are fragile cells with a short life span outside the body. Ways to reduce cell death in a biochemicalway are almost unknown due to scarce informationon the type of cellular death that is induced in fattissue. This study was designed to investigate the apoptoticpathways of fat tissue in a permanent perfusion bioreactorsystemwith the Hannover preservation solution and theEurocollins solution in fat flaps of rats.

Material und Methoden: In Lewis rats, the inguinal adipofascial flaps were elevated bilaterally andplaced in a bioreactor at 37°C. To detect caspases 3, 8, 9and 12, immunofluorescence stains of fat tissue specimenwere analysed at several time points after preservation offlaps were placed in Hannover solution and Eurocollinssolution for 10 days. An additional visual assessment ofviability by a calcein based life/dead test was performed.

Ergebnisse: It revealed a superior viability of the adipose tissue preservedin Hannover solution. Immunofluorescence staining demonstratedthat apoptotic pathways via mitochondria, endoplasmaticreticulum and death receptors were activated, asCaspases 8, 9 and 12 were detected. Caspase 3 as aneffector in the common apoptotic pathway was detected aswell. Adipose tissue preserved at 37°C ex vivo in a bioreactorsystem undergoes apoptosis. Immunofluorescenceexamination of the fat tissue preserved ex vivo revealedthat apoptotic pathways via mitochondria, endoplasmaticreticulum and death receptors are being activated.

Schlussfolgerung: Significantly less activation of Caspase 3, 8, 9 and 12 in flapspreserved in Hannover solution in comparison to Eurocollinswas found, supporting the anti apoptotic characteristicsof Hannover solution. Based on these findings,further research to modify the apoptotic pathways toameliorate viability of fat tissue can be performed.