Artikel
Apoptosewege im Fettgewebe
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Veröffentlicht: | 26. April 2013 |
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Gliederung
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Einleitung: Fat cells are fragile cells with a short life span outside the body. Ways to reduce cell death in a biochemicalway are almost unknown due to scarce informationon the type of cellular death that is induced in fattissue. This study was designed to investigate the apoptoticpathways of fat tissue in a permanent perfusion bioreactorsystemwith the Hannover preservation solution and theEurocollins solution in fat flaps of rats.
Material und Methoden: In Lewis rats, the inguinal adipofascial flaps were elevated bilaterally andplaced in a bioreactor at 37°C. To detect caspases 3, 8, 9and 12, immunofluorescence stains of fat tissue specimenwere analysed at several time points after preservation offlaps were placed in Hannover solution and Eurocollinssolution for 10 days. An additional visual assessment ofviability by a calcein based life/dead test was performed.
Ergebnisse: It revealed a superior viability of the adipose tissue preservedin Hannover solution. Immunofluorescence staining demonstratedthat apoptotic pathways via mitochondria, endoplasmaticreticulum and death receptors were activated, asCaspases 8, 9 and 12 were detected. Caspase 3 as aneffector in the common apoptotic pathway was detected aswell. Adipose tissue preserved at 37°C ex vivo in a bioreactorsystem undergoes apoptosis. Immunofluorescenceexamination of the fat tissue preserved ex vivo revealedthat apoptotic pathways via mitochondria, endoplasmaticreticulum and death receptors are being activated.
Schlussfolgerung: Significantly less activation of Caspase 3, 8, 9 and 12 in flapspreserved in Hannover solution in comparison to Eurocollinswas found, supporting the anti apoptotic characteristicsof Hannover solution. Based on these findings,further research to modify the apoptotic pathways toameliorate viability of fat tissue can be performed.