gms | German Medical Science

130. Kongress der Deutschen Gesellschaft für Chirurgie

Deutsche Gesellschaft für Chirurgie

30.04. - 03.05.2013, München

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The role of side-population of malignant melanoma in recurrence, metastasis and prognosis

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  • Qi Bao - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Xueqing Hu - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Zheren Shao - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Fangzhen Jiang - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Li Chen - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of GI Surgery, Hangzhou
  • Huan Qian - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Lu Wang - The Second Affiliated Hospital, Zhejiang University School of Medicine, Department of Plastic and Reconstructive Surgery, Hangzhou
  • Christiane J. Bruns - LMU München - Klinikum Großhadern, Chirurgische Klinik und Poliklinik, Munich
  • Peter J. Nelson - Klinikum der Universitaet Muenchen, Campus Innenstadt, Medizinische Klinik und Poliklinik IV, Arbeitsgruppe Klinische Biochemie, Munich
  • Hanno Niess - LMU München - Klinikum Großhadern, Chirurgische Klinik und Poliklinik, Munich
  • Yue Zhao - LMU München - Klinikum Großhadern, Chirurgische Klinik und Poliklinik, Munich

Deutsche Gesellschaft für Chirurgie. 130. Kongress der Deutschen Gesellschaft für Chirurgie. München, 30.04.-03.05.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13dgch164

doi: 10.3205/13dgch164, urn:nbn:de:0183-13dgch1649

Veröffentlicht: 26. April 2013

© 2013 Bao et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.

Gliederung

Text

Introduction: Melanoma is the most threatening skin cancer in human. The key point, which influences the prognosis most, is recurrence and metastasis of melanoma. Cancer stem cells (CSCs) are a sub-population of cancer cells, which are related to chemo-resistance, local cancer recur and distant metastasis. Multi-pathway-targeting anti-CSC therapy shed new light on melanoma patient-tailored treatment.

Material and methods: Murine melanoma cell line B16BL6 was investigated as chemo-sensitive and –resistant cell line. Standard chemo-therapy drug Doxirubicin (Dox) was added in cell culture medium. After 1-4 months chemo-drug treatment, side-population cells of B16BL6 were sorted by the Hoechst staining followed with FACS analysis. E-cadherin and β-catenin expression was assessed by immunocytochemistry.

Results: The morphology of B16BL6 was changed from “flagstone" appearance to fibroblast-like style as treated by Dox, and the chemo-resistant cells are full of melanin in cytoplasm. Side-population cells can be enriched in chemo-resistant cells, which increased from 0.96% to 4.7% within 1 month. Meanwhile, the Dox IC50 elevated from 148.91ng/ml to 377.62ng/ml. E-cadherin expressed mainly at cell-membrane area, and cytoplasm-nuclear translocation of β-catenin can be seen as chemo-sensitive to –resistant cells.

Conclusion: Chemo-resistance induced cancer stem-like cell – side-population cells which express EMT-related genes and pathways. We hypothesize that these cells are a source of treatment resistance in vivo and blocking their emergence may prevent treatment failure.