Artikel
De novo significant role of Pancreas-derived stem cells in the in vivo epidermalization of skin wounds
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Autoren
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H. Salem
- Dept. of Plastic Surgery, TU Munich, Germany.
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P. Ciba
- Fraunhofer institute for Marine Biotechnology (EMB), Campus Lübeck, Germany
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J.T. Egana
- Dept. of Plastic Surgery, TU Munich, Germany.
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D.H. Rapoport
- Fraunhofer institute for Marine Biotechnology (EMB), Campus Lübeck, Germany.
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M. Kadry
- Dept. of Plastic Surgery, Kasr Alainy Faculty of Medicine (KAFM), Cairo University, Egypt.
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K. Reithmayer
- Dept. of Dermatology, Venerology and Allergology, University of Lübeck, Germany.
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C. Kruse
- Fraunhofer institute for Marine Biotechnology (EMB), Campus Lübeck, Germany.
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H-G. Machens
- Dept. of Plastic Surgery, TU Munich, Germany.
| Veröffentlicht: | 23. April 2009 |
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Gliederung
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Introduction: In the rapidly growing field of stem cells, pancreas derived stem cells (PSCs) have been explored mainly with respect to their therapeutic potential in the treatment of diabetes. Different research groups have reported that PSCs differentiate not only in pancreatic cell types, but in a multitude of different cells, including ectodermal cell types. In this study, we present the first in vivo attempt to use PSCs in the field of skin regeneration.
Material and methods: A population of 5x105 PSCs from wistar rats were used to bioactivate 15 mm diameter circular Matriderm® sheets. Cells survival and proliferation were then tested in vitro after an over night incubation with the scaffolds to ensure their survival and homogenous distribution. Bioactivated scaffolds were then used to replace bilateral full-thickness skin wounds made on the dorsum of nude mice. A control group of nude mice received the Matriderm® scaffolds without cells. Two weeks after transplantation, the wound areas were harvested and analyzed with respect to epithelialization, vascularization and wound closure.
Results: After two weeks; the healing percentage and regeneration rate were significantly increased in the group of the PSCs-bioactivated scaffolds (factor of 2.1). Transillumination of the wound area and vessel quantification showed a significant increase in the vascularization percentage with the bioactivated scaffolds (factor of 1.5). Skin-like structures positive to epidermal markers (K14, K10 & Fillagrin) were evident in the healing wound bed via Morphology and Immunohistochemistry. PSCs were detected in the healing wound tissues using PCR. In vitro cytokine arrays in case of PSCs-bioactivated scaffolds showed a significant rise in the inflammatory cytokines responsible for immune defense (GM-CSF, IL-1 alpha and IL-6) together with the VEGF signaling for both vasculogenesis and angiogenesis.
Conclusion: In our study we prove the de novo role of PSCs in medicine. Their abilities extend beyond the local repair of insulin beta cells to entail the improvement in epidermalization percentage, vascularization and thus healing of full-thickness skin wounds.
