gms | German Medical Science

35. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2017)

11.01. - 14.01.2017, Chur, Schweiz

Characterization of burn injuries by monitoring immune and inflammatory response in the dermis with open flow microperfusion

Meeting Abstract

  • K. Tiffner - HEALTH - Institute for Biomedicine and Health Sciences, JOANNEUM RESEARCH Forschungsgesellschaft mbH, Graz, Austria
  • P. Wurzer - Division of Plastic, Esthetic and Reconstructive Surgery, Medical University of Graz, Graz, Austria
  • M. Funk - Bioskinco GmbH, Würzburg, Germany
  • L. Kamolz - Division of Plastic, Esthetic and Reconstructive Surgery, Medical University of Graz, Graz, Austria
  • T. Birngruber - HEALTH - Institute for Biomedicine and Health Sciences, JOANNEUM RESEARCH Forschungsgesellschaft mbH, Graz, Austria

Deutschsprachige Arbeitsgemeinschaft für Verbrennungsbehandlung. 35. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2017). Chur, Schweiz, 11.-14.01.2017. Düsseldorf: German Medical Science GMS Publishing House; 2017. Doc17dav10.8

doi: 10.3205/17dav87, urn:nbn:de:0183-17dav871

Veröffentlicht: 18. Januar 2017

© 2017 Tiffner et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

The immune and inflammatory reaction after burn injuries is standardly measured in blood serum to optimize therapeutic treatments. In this project we aimed to investigate the local reaction in the dermis after a burn stimulus which is insufficiently studied due to a lack of appropriate sampling methods. Open flow microperfusion (OFM) enables sampling of the interstitial fluid (ISF) directly in the dermal tissue and can thus be used to investigate the inflammatory reaction in response to a burn stimulus applied to the skin surface.

The minimally invasive OFM technique enables continuous sampling of pure and diluted ISF in the tissue of interest (e.g. dermis, adipose tissue).The OFM probe (OD: 0.5 mm) features a linear design and macroscopic openings at the exchange area form the interface to the tissue at a length of 15 mm. The CE-certified OFM probe is available for preclinical and clinical studies and also ex-vivo studies using excised human skin samples to continuously collect ISF for up to 46 hours. By using continuous perfusion of the OFM probe with a perfusion fluid, ISF can be sampled and analyzed regarding inflammation markers. Changes in the skin penetration ability of substances due to pathophysiological changes caused by a thermic burn stimulus can also be investigated.

This setup offers new experimental possibilities for the investigation of burn injuries and the characterization of different burn injuries for an optimization of therapeutic treatments.