Artikel
Morphological findings of experimental pig models with outer retinal degeneration induced by intravitreal loading of Nmethyl-N-nitrosourea after vitrectomy
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Autoren
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Seong-woo Kim
- Department of Ophthalmology, Korea University College of Medicine, Seoul/ROK
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K.-E. Choi
- Department of Ophthalmology, Korea University College of Medicine, Seoul/ROK
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Y. S. Goo
- Department of Physiology, Chungbuk National University School of Medicine, Cheongju/ROK
| Veröffentlicht: | 10. Dezember 2019 |
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Gliederung
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Objective: To develop and characterize the experimental pig models with outer retinal degeneration induced by intravitreal loading of an N-methyl-N-nitrosourea (MNU) after vitrectomy. Materials and methods: Various dose of MNU were temporally loaded and fully removed respectively in the left or right eyes of female micropig after vitrectomy. To identify morphological changes of retina and spectral-domain optical coherence tomography (OCT) were respectively performed during follow up periods after the exposure of MNU. Histological examinations with hematoxylin and eosin (H&E) stain were performed on selected pig eyes at last observation period after the exposure to MNU. Electroretinography (ERG) including multifocal ERG were also performed at baseline, and follow-up periods after the exposure to MNU.
Results: After temporary MNU loading during vitrectomy, both complete and incomplete cases for outer retinal degeneration pig model were successfully induced. In complete cases, the loss of ellipsoid zone and outer nuclear layer were observed in OCT. ERG in complete cases showed signal flattening in both full field ERG and multifocal ERG. Histology in complete cases showed the complete loss of photoreceptors and outer retinal cells. In incomplete cases, no typical loss of ellipsoid zone and outer nuclear layer were observed in OCT, but there were also significant change of total retinal thickness and outer nuclear layer thickness. ERG in incomplete cases showed decreased amplitudes or delayed implicit time compared with baseline ERG results. Histologic findings also showed decreased number of photoreceptors and outer retinal cells.
Discussion: Experimental pig models with outer retinal degeneration can be completely or incompletely induced by temporally loading for optimal dose of MNU after vitrectomy.
Acknowledgment: This research was supported in part by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2016R1D1A1A02937018) and the Biog & Medical Technology Development Program of the NRF funded in part by the Korean government, the Ministry of Science and ICT (MSIP) (NRF-2017M3A9E2056458 and 2017M3A9E2056460).
