gms | German Medical Science

7th International Symposium on AMD: Age-related Macular Degeneration – Understanding Pathogenetic Mechanisms of Disease

20.09. - 21.09.2019, Baden-Baden

In vivo photoreceptor imaging by non-adaptive optics confocal scanning laser ophthalmoscopy

Meeting Abstract

  • Thomas Theelen - Radboud University Medical Center, Nijmegen/NL
  • P.P.A. Dhooge - Radboud University Medical Center, Nijmegen/NL
  • T.W.F. Mulders - Radboud University Medical Center, Nijmegen/NL
  • C.B. Hoyng - Radboud University Medical Center, Nijmegen/NL

7th International Symposium on AMD: Age-related Macular Degeneration - Understanding Pathogenetic Mechanisms of Disease. Baden-Baden, 20.-21.09.2019. Düsseldorf: German Medical Science GMS Publishing House; 2020. Doc19amd52

doi: 10.3205/19amd52, urn:nbn:de:0183-19amd522

Veröffentlicht: 5. Februar 2020

© 2020 Theelen et al.
Dieser Artikel ist ein Open-Access-Artikel und steht unter den Lizenzbedingungen der Creative Commons Attribution 4.0 License (Namensnennung). Lizenz-Angaben siehe http://creativecommons.org/licenses/by/4.0/.


Gliederung

Text

Purpose: Retinal photoreceptor (PR) imaging has been proven to be possible by adaptive optics (AO) systems. Here, we aim to show the mosaic of human PRs in vivo by a non-AO system.

Methods: Healthy subjects and patients with macular disease were examined by a Heidelberg Engineering Spectralis™ device equipped with the High Magnification Module (HMM)™. Besides standard 30⁰ near-infrared confocal scanning laser opthalmoscopy (CSLO), we performed spectral domain optical coherence tomography (OCT) and 8⁰ square CSLO imaging with HMM™.

Results: Two healthy subjects and four patients with macular diseases (Dominant Cystoid Macular Dystrophy, Adult Vitelliform Macular Dystrophy (AVMD), retinitis Pigmentosa (RP), macular pucker) were examined. Retinal PR mosaics could be observed and analyzed in all HMM™ images. In healthy subjects and the macular pucker case, retinal PRs appeared homogeneous and equally distributed in the macular area. In AVMD and RP, the PR mosaic was less homogeneous with a reduced number of PRs and changed optical properties. Areas of reduced PR density or optical changes on HMM™ images also showed alterations or loss of the outer retinal bands on OCT images.

Conclusion: In vivo PR imaging in healthy and diseased retinas appeared possible by a non-AO device, the Heidelberg Engineering Spectralis™ system with HMM™. A major advantage was the enlarged field of view in HMM™ as opposed to AO. Within the system, image registration of HMM™ images allowed for precise localization of observed changes and correlation with other imaging modalities like OCT.