gms | German Medical Science

Purpose: To determine clinical,biological,morphology-structural elements as biomarkers for AMD and its Atrophy and Neovascular complications

Methods: AMD: 320 patients: groupA: 142, with AMD drusenoid deposits “L”,Lipid Type and “P”,Protein-cellular type; groupB: 64, with Atrophy complication; group C :114, with Neovascular complication. Complete Ophthalmologic exam: visual acuity,Fundus examination, Multimodal imaging(FAF,OCT,OCTen face). Morphology-Structural software(M-S software): analyze drusenoid deposit (volume, contours, 3D), let contents analyze, discrimination, differentiation, let grading, measurements(volume, density, structure), so “L” and “P” deposits evaluation, characterization. Cognitive evaluation: with Mini Mental State Examination, for all patients, score allow to determine various subgroups. Lipidomic Study: Blood tests and analysis, all lipids qualitative, quantitative analysis, all the same for all patients. Blood test is done during ophthalmologic exam. Plasma congelation“snap frost”after total blood centrifugation,then liquid-liquid extraction for lipids analysis:neutral lipid,fatty acid, phospholipids,as sphingolipids,Polyinsatured fatty acids too.

Results: Mild cognitive impairment (MCI) for 73% patients in group A, 77,5% group B,63% group C. In MCI subgroups: progressive decrease and similar in group A and B, lower and homogeneous in group B. Cognitive impairment differ between AMD subgroups: moderate in group A, higher in group B and C, and more in group B than in group C, but early AD only present in group C. Multi modal, M-S Software well characterize, individualize drusenoid deposits “L” and “P” and their evolution which is different. Lipidomics: similar Results for group A and C for Total Neutral Lipids,Fame,Free Fatty acid,Oxysterols,Phospholipids. Significative difference for group C with Eicosanoids,Fame free fatty acids; for group B with sphingosins,total neutral lipids,phospholipids total and Phosphatidyl choline; for group A with Oxysterol. Results are effective, statistically significative. So, Cognitive exam, Multi modal, M-S Software, Lipidomics become and are Biomarkers for AMD and its complications, allow better AMD follow-up and etiopathogenesis understanding.

Conclusion: Each clinical,biological,multimodal entity,all,together or not,are Biomarkers,allow AMD screening,follow-up.They also lead to better etiopathogenesis understanding and therapeutics prospects