gms | German Medical Science

26th Annual Meeting of the German Retina Society

German Retina Society

27.09.2013, Hamburg

The concentration dependent effects of indocyanine green on retinal function in the electrophysiological ex-vivo model of the isolated perfused vertebrate retina

Meeting Abstract

  • Matthias Lüke - University Eye Hospital, University of Lübeck, Germany
  • M. Ranjbar - University Eye Hospital, University of Lübeck, Germany
  • A. Tapenbayeva - University Eye Hospital, University of Lübeck, Germany
  • A. Tura - University Eye Hospital, University of Lübeck, Germany
  • S. Grisanti - University Eye Hospital, University of Lübeck, Germany
  • T. Schneider - Institute of Neurophysiology, University of Cologne, Germany; Center of Molecular Medicine Cologne, University of Cologne, Germany
  • J. Lüke - University Eye Hospital, University of Lübeck, Germany

Retinologische Gesellschaft. 26. Jahrestagung der Retinologischen Gesellschaft. Hamburg, 27.-27.09.2013. Düsseldorf: German Medical Science GMS Publishing House; 2013. Doc13rg05

doi: 10.3205/13rg05, urn:nbn:de:0183-13rg055

Published: August 20, 2013

© 2013 Lüke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Background: Dye solutions such as indocyanine green (ICG) are used for intraoperative staining of epiretinal membranes and the internal limiting membrane to enhance the visualization and to facilitate their removal. The aim of the presented study was to investigate the effects of ICG on bovine retinal function using different concentrations of ICG.

Methods: Bovine retina preparations were perfused with a standard solution and the electroretinogram (ERG) was recorded. After recording stable ERG-amplitudes the nutrient solution was substituted by an ICG solution with varying concentrations (0.000025%, 0.00025%, 0.0025% & 0.025%) for 45 minutes. Afterwards the preparations were reperfused with standard solution for at least 85 minutes. The percentage of b-wave reduction was measured after exposure with ICG and at the end of the washout.

Results: Significant reductions (p<0.05) of the b-wave amplitude were found for concentrations of 0.0025% (p=0.00985) and 0.025% (p=0.03775) ICG after exposure. But while for the concentration of 0.025% ICG the b-wave amplitude remained significantly decreased (p=0.0082) after the observation period, a full recovery of the b-wave was observed for the concentration of 0.0025% (p=0.19171). The lower tested concentrations of ICG did not significantly affect the bwave amplitude during the exposure as well as at the washout.

Conclusion: The effects of ICG after retinal exposure depend inter alia on the applied concentrations. The intraocular application of sufficient ICG concentrations for ILM-staining seems not possible without interfering with retinal function.