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Infektiologie Update 2016: 25. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG)

Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG)

06.-08.10.2016, Rostock

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Indirect multiplex enzyme-linked immunosorbent assay for the detection of Chlamydia, Borrelia, and Ehrlichia in axial spondyloarthritis

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  • author Louise Thuesen Hermansen - King Christian 10th Hospital for Rheumatic Diseases, Gråsten, Denmark; Institute for Regional Health Research, University of Southern Denmark, Denmark
  • K. Garg - Department of Biology and Environmental and Nano Science, University of Jyväskylä, Finland
  • K. Karvonen - Department of Biology and Environmental and Nano Science, University of Jyväskylä, Finland
  • A. G. Loft - Department of Medicine, Hospital Lillebælt, Vejle, Denmark; Department of Rheumatology, Aarhus University Hospital, Denmark
  • T. Barington - Department of Clinical Immunology, Odense University Hospital, Denmark
  • A. A. Christiansen - King Christian 10th Hospital for Rheumatic Diseases, Gråsten, Denmark; Institute for Regional Health Research, University of Southern Denmark, Denmark
  • B. Arnbak - Institute for Regional Health Research, University of Southern Denmark, Denmark; Research Department Spine Centre of Southern Denmark, Middelfart, Denmark
  • K. Hørslev-Petersen - King Christian 10th Hospital for Rheumatic Diseases, Gråsten, Denmark; Institute for Regional Health Research, University of Southern Denmark, Denmark
  • O. Hendricks - King Christian 10th Hospital for Rheumatic Diseases, Gråsten, Denmark; Institute for Regional Health Research, University of Southern Denmark, Denmark
  • L. Gilbert - Department of Biology and Environmental and Nano Science, University of Jyväskylä, Finland

Infektiologie Update 2016. 25. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG). Rostock, 06.-08.10.2016. Düsseldorf: German Medical Science GMS Publishing House; 2016. Doc16peg22

doi: 10.3205/16peg22, urn:nbn:de:0183-16peg228

Published: September 30, 2016

© 2016 Hermansen et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. See license information at http://creativecommons.org/licenses/by/4.0/.

Outline

Text

In this proof-of-concept study we assessed the presence of circulating IgG and IgM antibodies against Chlamydia pneumonia and Chlamydia trachomatis together with Borrelia burgdorferi, Borrelia afzelii, Borrelia garinii, and Ehrlichia in sera from patients classified as having axial spondyloarthritis (axSpA) and blood donors (BD).

An inhouse developed multiplex enzyme-linked immunosorbent assay (ELISA) was developed to study 40 axSpA patients and 40 BDs. Novelty of this assay was inclusion of the detection of antibodies against species-specific peptides from Chlamydia pneumoniae and Chlamydia trachomatis.

Based on positive tests for both Chlamydia species and IgG and IgM antibody isotypes, sensitivity and specificity of the assay was 75% and 88.5%, respectively. Increased prevalence of IgG and IgM antibodies in axSpA patients compared with BDs were observed against C. pneumoniae (20% and 12.5% for IgG and IgM respectively), B. burgdorferi (40%, 35%), B. afzelii (32.5%, 15%), B. garinii (50%, 20%), and Ehrlichia (45%, 30%). In addition, increased prevalence of IgG in axSpA patients were observed for C. trachomatis (17.5%).

The introduction of speciesspecific peptides from C. pneumoniae and C. trachomatis into an ELISA allowed for polymicrobial detection of antibodies against five microorganisms relevant for the phenotypical characterization of axSpA, simultaneously. The results support the conclusion that previous infections may play a role in the pathogenesis of axSpA and the new assay may be relevant in persistent LD in order to detect relevant coinfections.

Figure 1 [Fig. 1], Figure 2 [Fig. 2]

Outline

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