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85th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

28.05. - 01.06.2014, Dortmund

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Imaging of the human inner ear with scanning laser optical tomography (SLOT)

Meeting Abstract

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  • corresponding author Saleh Mohebbi - tehran university of medical scence,international, tehran, iran
  • Thomas Rau - Otoraryngology-VIANA, Hannover
  • Heiko Meyer - HNO-Klinik, MH, Hannover
  • Thomas Lenarz - HNO-Klinik, MH, Hannover
  • Omid Majdani - HNO-Klinik, MH, Hannover

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 85. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Dortmund, 28.05.-01.06.2014. Düsseldorf: German Medical Science GMS Publishing House; 2014. Doc14hnod431

doi: 10.3205/14hnod431, urn:nbn:de:0183-14hnod4318

Published: April 14, 2014

© 2014 Mohebbi et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: SLOT is a fluorescence microscopy technique allowing scanning of samples of a size of several millimeters, thus enabling volumetric (3D) visualization by using intrinsic contrast mechanisms of previously perpetrated and decalcified bony labirynth.

Methods: Three explanted temporal bones were prepared for SLOT imaging. The dissection included decalcification in an EDTA solution as well as drilling the boney structure surrounding the cochlea and labyrinth. The membranous labyrinth is than fixed into the laser scanning device, producing translucent 3D images showing the anatomy of the intra cochlear and intra labyrithal microstructures.

Result: The method allows analyzing nondestructively and quantitatively in three-dimensional datasets in any preferred planar orientation. 2D and 3D images of the cochlea can demonstrate the intactness of the membranous structures and will be demonstrated.

Conclusion: In further steps we plan to perform SLOT imaging after implanting cochlear implant electrodes into the cochlea in residual hearing preservation technique and examine the intra cochlear structures for any violation of the microstructure.

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