gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

Amniotic membrane transplantation for persistent corneal epithelial defects in eyes after penetrating keratoplasty

Meeting Abstract

  • corresponding author R. Sauer - University Erlangen-Nürnberg, Erlangen
  • B. Seitz - University Erlangen-Nürnberg, Erlangen
  • S. Das - University Erlangen-Nürnberg, Erlangen
  • D. Mena - University Erlangen-Nürnberg, Erlangen
  • C. Hofmann-Rummelt - University Erlangen-Nürnberg, Erlangen
  • A. Langenbucher - University Erlangen-Nürnberg, Erlangen
  • F. E. Kruse - University Erlangen-Nürnberg, Erlangen

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogP 032

The electronic version of this article is the complete one and can be found online at:

Published: September 22, 2004

© 2004 Sauer et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.




To determine the primary success and recurrence rate of amniotic membrane transplantation (AMT) for treatment of persistent corneal epithelial defects (PEDs) in eyes after one or more previous penetrating keratoplasties (PKs).


AMT was performed in 24 eyes of 24 patients for erosions without stromal defect (n=6) and ulcers (n=18), which were resistant to medical therapy. All the patients had undergone one (42%) or more PKs prior to AMT. After removal of the epithelium and pannus (if present), one or more layers of AM depending on the depth of the lesion were fixed with interrupted 10-0 nylon suture. Three different surgical techniques were performed: 'inlay' or 'graft' (n=3), 'overlay' or 'patch' (n=5), and 'sandwich' or combination of 'graft' and 'patch' (n=16). Main outcome measures included surgical success (SS), if the epithelium was closed within four weeks after AMT, and recurrence (R), if a new epithelial defect developed during follow-up (15 ± 12 months) after the complete epithelial closure.


The rate of SS was 70% (erosions: 80%, ulcers: 67%) and was found to be inversely proportional to the number of previous PKs. Moreover, the defects limited only to the centre of the corneal graft had a higher success rate (central: 100%, non-central: 61%). The diseases did not differ essentially for both SS and primary failure groups. Seven out of sixteen successful eyes (44%; erosions: 75% vs. ulcers: 33%) had a R after a mean follow-up of 16 ± 13 months. The rate of SS was highest (81%) and the rate of R was lowest (38%) with the 'sandwich' technique.


AMT may be beneficial in the treatment of PEDs after PK. Especially when the 'sandwich' technique is applied, the primary success rate is comparable to that without previous PK. In contrast, recurrences seem to be more frequent after PK. Treatment of systemic or other associated ocular diseases is indispensable for success.