gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

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Purinergic modulation of human Schlemm's canal and trabecular meshwork cells

Meeting Abstract

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  • corresponding author M. O. Karl - University of Pennsylvania, Department of Physiology, Philadelphia, USA
  • K. Peterson-Yantorno - University of Pennsylvania, Department of Physiology, Philadelphia, USA
  • R. A. Stone - University of Pennsylvania, Department of Physiology, Philadelphia, USA
  • M. M. Civan - University of Pennsylvania, Department of Physiology, Philadelphia, USA

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogDO.14.01

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog122.shtml

Published: September 22, 2004

© 2004 Karl et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

Adenosine can lower IOP in humans. A1 adenosine receptor (AR) agonists can lower and A2a increase IOP in several species. Main target sites and underlying mechanisms are unknown. Concentrations of aqueous humor adenosine are increased in patients with primary open-angle glaucoma and PEX glaucoma.

Methods

We applied selective AR agonists to human Schlemm's canal cells and a human trabecular meshwork cell line (hTM5) to evaluate the effects on whole-cell current and to determine whether cell volume is altered. Inner-wall SC (IW-SC) cells were isolated by a novel enzyme-assisted technique, followed by cell selection with antibody to the SC-cell marker CD31/PECAM-1. Cells grown on coverslips were loaded with calcein-AM and Pluronic F-127 to measure cell area as an index of cell volume by videomicroscopy. Whole-cell currents were recorded using patch-clamp technique.

Results

The A1AR agonist S-ENBA increased IW-SC whole-cell currents (100nM, 18/22 applications, 718±232%). In contrast the A2AAR agonist CGS 21680 reduced currents (30nM, 4/7, -29±7%). Contrary hTM5 cells responded to all subtype-specific AR agonists tested with increased currents. Only the A1AR agonist S-ENBA shrank the inner-wall SC cells (-2.4±0.1%, N=8) and only the A2AAR agonist CGS 216380 shrank the hTM5 cells (-3.1±0.1%, N=7).

Conclusions

IW-SC cells can be isolated by a novel technique. All AR agonists tested alter inner-wall SC cells as well as hTM5 cells whole-cell current. Opposing actions of A1 and A2a AR agonists on IW-SC whole-cell currents suggest IW-SC may mediate IOP modulation.