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Joint German Congress of Orthopaedics and Trauma Surgery

02. - 06.10.2006, Berlin

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BMP-2 shows high angiogenetic potential - An in vitro and in vivo study

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  • V. Alt - Klinik und Poliklinik für Unfallchirurgie, Universitätsklinikum Giessen-Marburg, Standort Giessen, Giessen, Germany
  • D. Schneider - Medizinische Klinik, Universität Heidelberg, Heidelberg, Germany
  • M. Obert - Neuroradiologie, Universitätsklinikum Giessen-Marburg, Standort Giessen, Giessen, Germany
  • H. Traupe - Neuroradiologie, Universitätsklinikum Giessen-Marburg, Standort Giessen, Giessen, Germany
  • S. Wenisch - Labor für Experimentelle Unfallchirurgie, Universitätsklinikum Giessen-Marburg, Standort Giessen, Giessen, Germany
  • R. Schnettler - Klinik und Poliklinik für Unfallchirurgie, Universitätsklinikum Giessen-Marburg, Standort Giessen, Giessen, Germany

Deutscher Kongress für Orthopädie und Unfallchirurgie. 70. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 92. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie und 47. Tagung des Berufsverbandes der Fachärzte für Orthopädie. Berlin, 02.-06.10.2006. Düsseldorf, Köln: German Medical Science; 2006. DocE.4.1-1808

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgu2006/06dgu0107.shtml

Published: September 28, 2006

© 2006 Alt et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

BMP-2 has osteoinductive properties und leads to de novo bone formation. Furthermore, BMP-2 leads to improved soft tissue healing and reduction of infection rates for which pro-angiogenetic effects of BMP-2 are assumed. The purpose of this study was to evaluate the angiogenetic properties of BMP-2.

A sprouting test with bovine retinal endothelial cells (BREC-assay) was used to determine the angiogenetic potential of BMP-2 in vitro in comparison to VEGF and to negative controls.

Furthermore, growth factor-loaded matrigels were injected subcutaneoulsy in mice. Two different doses of BMP-2 (100 ng/ml and 500 ng/ml) were tested. After 6 days volumetric computer tomography scanning of the mice was performed to assess new blood vessel formation in the gel itself and of the surrounding area. Afer CT scanning the gels were explanted, homogenized and the hemoglobin content of the gels was determined as indirect hint for the amount of new blood vessel formation.

Both the BREC and the matrigel assay showed a significant enhanced new blood vessel formation by BMP-2 compared to the negative controls. The angiogenetic potential of the 500 ng/ml BMP-2 dose was about 80% of the activity of VEGF in the BREC assay. Volumetric CT scanning showed newly formed blood vessels originating from the farer environment which subsequently transformed into a vascular network within the gel which was not the case in the negative controls.

The current study shows that BMP-2 exhibits angiogenetic activity which might be related to its osteoinductive properties.