gms | German Medical Science

57th Annual Meeting of the German Society of Neurosurgery
Joint Meeting with the Japanese Neurosurgical Society

German Society of Neurosurgery (DGNC)

11 - 14 May, Essen

Anatomy of the P1-Segment has no effect on cerebral perfusion after proximal vessel occlusion in C57/BL6 mice

Die Anatomie des P1-Segments hat keinen Einfluss auf die zerebrale Perfusion nach proximaler Gefäßokklusion in C57/BL6 Mäusen

Meeting Abstract

  • corresponding author N. Hecht - Neurochirurgische Klinik, Universitätsklinik Mannheim, Mannheim
  • P.G. Peña-Tapia - Neurochirurgische Klinik, Universitätsklinik Mannheim, Mannheim
  • J. Woitzik - Neurochirurgische Klinik, Universitätsklinik Mannheim, Mannheim
  • P. Vajkoczy - Neurochirurgische Klinik, Universitätsklinik Mannheim, Mannheim

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. Essen, 11.-14.05.2006. Düsseldorf, Köln: German Medical Science; 2006. DocP 10.158

The electronic version of this article is the complete one and can be found online at:

Published: May 8, 2006

© 2006 Hecht et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.



Objective: A strong interest in the use of transgenic mice for stroke research has developed. However, the induction of acute or chronic ischemia has been hampered by the excellent collateral blood supply in mice. Recently, the contribution of the P1-Segment of the posterior cerebral artery to this collateralization has been specifically discussed. Therefore, the aim of this study was to analyse the impact of the P1 anatomy on cerebral perfusion following different types of vessel occlusion in C57/BL6 mice, which is the background of most transgenic mouse strains.

Methods: 30 male C57/BL6 mice, aged 10 to 12 weeks (22g – 28g bw) were anaesthetized with Ketamine/Xylazin. For cerebral blood flow (CBF) measurement, laser-Doppler flowmetry probes were bilaterally mounted over the intact parietal skull. One, 2-, 3- or 4 vessel occlusion (VO) was performed by unilateral or bilateral occlusion of the common carotid artery (CCA) with or without bilateral vertebral artery thermo-coagulation. To obtain cerebral angiograms for P1 anatomy assessment, mice were sacrificed and perfused with black liquid latex (38°C) at a perfusion pressure of 150 mmHg. For maximal vessel dilatation, papaverin was administered in a dosage of 50 mg / kg prior to perfusion.

Results: After unilateral CCA occlusion we observed an ipsilateral CBF drop to 82±9% of baseline. Two, 3 and 4 VO resulted in a decline to 77±10%, 37±11% and 16±8%, respectively. However, we were not able to show any difference in CBF drop when comparing the groups consisting of mice with none, unilateral or bilateral presence of the P1.

Conclusions: Although the anatomy of the P1-Segment may be highly heterogeneous, varying from no to bilateral connections between the anterior and posterior circulation, it has no effect on the perfusion consequences following proximal vessel occlusion.