Article
Co-expression pattern of growth factor receptors in a model of wound healing for esophagogastric anastomoses in rats
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Published: | April 26, 2013 |
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Introduction: Anastomotic leakage after gastrointestinal surgery is a significant cause of morbidity and mortality. In particular, esophagogastric anastomoses are vulnerable to leakage, resulting in an increased need for reoperation and a high risk of subsequent anastomotic stenosis formation and fistula. Studies in well-established experimental rodent models showed a positive impact of growth factors on anastomotic wound healing. So far, methodic investigations on the expression profile of growth factor receptors in the gastrointestinal tract do not exist.
Material and methods: We investigated the co-expression pattern of vascular growth factor receptor (VEGFR1-3), epidermal growth factor receptor (EGFR1), platelet-derived growth factor receptor (PDGFRα/β) and keratinocyte growth factor receptor (KGFR) in a model of wound healing for esophagogastric anastomoses in the rat by RT-PCR analysis.
Results: VEGFR1-3, EGFR1, PDGFRα/β und KGFR expression in esophageal and gastric samples showed strong to intermediate intensity comparing with samples of small intestine and colon.
Conclusion: This is the first experimental study showing a wide expression of a series of potential growth factor receptors in the rat intestine. It will be interesting to evaluate anastomotic healing by local administration of these growth factors in our established model of wound healing for esophagogastric anastomoses in rats.