Article
Fresh mesothelial lesions highly attract free floating intraperitoneal colorectal tumour cells in a rat model
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Published: | April 23, 2009 |
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Introduction: Intraperitoneal free cancer cells are associated with a higher risk of recurrence and a poorer prognosis in colorectal surgery. Tumour recurrence may occur early after surgery. One potential mechanism is the ability of peritoneal lesions to attract tumour cells.
Material and methods: In Wag-Rija rats, the parietal peritoneum was resected on a defined area and a corresponding control area was marked with 4 sutures in the same rat. Colorectal tumor cells (CC531, 2.5x106) were injected into the abdominal cavity at the end of surgery. The abdominal wall was harvested at 6 or 9 days and tissue samples (peritonectomy and control area, n=4 each group) were resected with a punch (diameter 2cm). The specimens were evaluated for weight, macroscopic tumor growth, and histological tumor infiltration.
Results: The weight of the abdominal wall was significantly increased in the area of peritonectomy after 6 and 9 days (control vs. peritonectomy [mg]; 6 days: 804±61 vs. 1001±20*; 9 days: 1348±145 vs .2168±296*;*p<0.05). Tumor growth was significantly increased in the area of peritonectomy (control vs. peritonectomy [%]; 6 days: 11±5 vs. 64±10*; 9 days: 39±11 vs. 88±5*;*p<0.002).The tumor depth was more than 3.8 fold increased in the area of peritonectomy (control vs. peritonectomy [mm]; 6 days: 2.19±0.26 vs. 0.82±0.14*; 9 days: 1.55±0.4 vs. 5.96±1.4*;*p<0.01).
Conclusion: Peritoneal defects may play an important role in the pathogenesis of tumour implantation and might have some impact on tumour recurrence. The peritoneal damage should be kept as low as possible in patients undergoing tumour resection.