gms | German Medical Science

30. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2012)

11.01. - 14.01.2012, Nassfeld, Österreich

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Improvement of skin quality using a new collagen scaffold in acute burns and reconstructive surgery: An in vivo evaluation of split skin graft transplantations in a rat model

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  • corresponding author J. Schiefer - Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Berufsgenossenschaftliche Unfallklinik, Eberhard-Karls Universität Tübingen
  • M. Held - Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Berufsgenossenschaftliche Unfallklinik, Eberhard-Karls Universität Tübingen
  • T. Knoeller - Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Berufsgenossenschaftliche Unfallklinik, Eberhard-Karls Universität Tübingen
  • H.-E. Schaller - Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Berufsgenossenschaftliche Unfallklinik, Eberhard-Karls Universität Tübingen
  • A. Rahmanian-Schwarz - Klinik für Hand-, Plastische-, Rekonstruktive- und Verbrennungschirurgie, Berufsgenossenschaftliche Unfallklinik, Eberhard-Karls Universität Tübingen

Deutschsprachige Arbeitsgemeinschaft für Verbrennungsbehandlung. 30. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2012). Nassfeld, Österreich, 11.-14.01.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12dav65

doi: 10.3205/12dav65, urn:nbn:de:0183-12dav655

Published: August 7, 2012

© 2012 Schiefer et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Despite the wide establishment of split thickness skin grafting for the reconstruction of tissue defects, it is often associated with a reduction of skin quality due to hypertrophic scarring. In this context a new collagen cell carrier (CCC) as an underlayment in split skin graft transplantation for covering tissue defects in rats has been evaluated as an improving agent.

Methods: In 28 laboratory rats full thickness wounds were covered with split skin autografts (SSG), applying CCC as an underlayment in treatment group (14 animals) versus SSG transplantation solitarily (14 animals). The objective assessment of the post grafting skin areas in terms of skin elasticity was realized by the application of the Cutometer MPA 580. Three parameters were considered for skin elasticity analysis: R0 (total deviation of the skin), R2 (gross elasticity), R8 (viscoelasticity). Measurements were performed every 10 days until day 80 postgrafting. Subsequently, biopsies for histological evaluation were taken.

Results: The results achieved in cutometer analysis recorded significantly superior elasticity values in CCC supplemented SSG regarding gross elasticity and viscoelasticity (R2/R8) starting from day 60 postgrafting up to the end of the measuring period. There was no histological evidence of inflammation, adverse host tissue reaction or scar tissue formation.

Conclusion: As split skin grafting is associated with dermal quality reduction, the beneficial effects of CCC in terms of skin graft quality improvement may offer unique opportunities in complex wound management.