gms | German Medical Science

27. Jahrestagung der Deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung (DAV 2009)

14.01. bis 17.01.2009, Leogang, Österreich

Matriderm as a scaffold for adipose tissue engineering: First results of an in vitro study with pretreated and non-treated human preadipocytes

Meeting Abstract

  • M. Keck - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria
  • D. Haluza - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria
  • D. Lumenta - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria
  • S. Burjak - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria
  • L. P. Kamolz - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria
  • M. Frey - Vienna Burn Centre, Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University Vienna, Austria

DAV 2009. 27. Jahrestagung der deutschsprachigen Arbeitsgemeinschaft für Verbrennungsbehandlung. Leogang, Österreich, 14.-17.01.2009. Düsseldorf: German Medical Science GMS Publishing House; 2009. Doc09dav21

DOI: 10.3205/09dav21, URN: urn:nbn:de:0183-09dav216

Published: March 19, 2009

© 2009 Keck et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.


Outline

Text

Background: Millions of plastic and reconstructive surgical procedures are performed each year to repair soft tissue defects that result from significant burns, tumor resections and congenital defects.

Tissue-engineering strategies are being investigated to develop methods for generating soft tissue. Preadipocytes represent a promising autologous cell source for adipose tissue engineering. These immature precursor cells, that are located between mature adipocytes in adpiose tissue, are much more resistant to mechanical damage and ischemic conditions than mature adipocytes.

In order to use preadipocytes for tissue engineering purposes, cells are isolated from human adipose tissue, cultured and seeded onto scaffolds.

Aim of the present study, was to determine, if a bovine derived collagen matrix with an elastin component (Matriderm®) could serve as a carrier for preadipocytes in-vitro.

Once processed preadipocytes become subject to the human tissue act and require handling under much tighter regulations. Therefore, we intended to identify any influence caused by induction of preadipocytes prior to seeding on adipose tissue formation.

Material and methods: Human preadipocytes were isolated from subcutaneous adipose tissue obtained from discarded tissue during abdominoplasty from healthy men and women. Preadipocytes were divided into three groups. Group I was seeded onto the scaffold directly after isolation, cells of group II were proliferated for 4 days before seeding and group III was proliferated and induced to differentiate before seeded onto the scaffold. A three dimensional scaffold (Matriderm®) containing bovine collagen and elastin served as a carrier. 21 days after seeding all scaffolds were histologically evaluated, using hematoxylin and eosin as well as immunofluorescence labeling with Pref-1 antibody (DLK (C-19)) and DAPI (4‘,6-diamidino- 2-phenylindole).

Results: Cells of all groups adhered to the scaffolds on day 21 after seeding. Cells of group 1 (freshly isolated preadipocytes) and 2 (proliferated preadipocytes) adhered well and penetrated into deeper layers of the matrix. In group 3 (induced preadipocytes) penetration of cells was primarily observed to the surface area of the scaffold.

Discussion/ Conclusion: The collagen-elastin matrix serves as a useful scaffold for adipose tissue engineering.

Freshly isolated preadipocytes as well as proliferated preadipocytes show good penetration into deeper layers of the scaffold, whereas induced preadipocytes attached primarily to the surface of the matrix.