gms | German Medical Science

29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

Deutsche Hochdruckliga e. V. DHL ® - Deutsche Hypertonie Gesellschaft Deutsches Kompetenzzentrum Bluthochdruck

23. bis 25.11.2005, Berlin

Signal transduction of the renin/ prorenin receptor

Signaltransduktion des Renin/ Prorenin Rezeptors

Meeting Abstract

Suche in Medline nach

  • H. Funke-Kaiser - Campus Charité-Mitte, Charité Berlin (Berlin,D)
  • J.H. Schefe - Campus Charité-Mitte, Charité Berlin (Berlin,D)
  • T. Unger - Campus Charité-Mitte, Charité Berlin (Berlin,D)

Hypertonie 2005. 29. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Berlin, 23.-25.11.2005. Düsseldorf, Köln: German Medical Science; 2006. Doc05hochP58

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2005/05hoch058.shtml

Veröffentlicht: 8. August 2006

© 2006 Funke-Kaiser et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

A human renin/ prorenin receptor (RER), which can specifically bind renin and prorenin, has recently been cloned. This receptor, which is highly expressed, in heart and brain, exerts a dual function: First, binding of renin to the RER increases renin's catalytic activity, and the receptor is able to unmask the catalytic activity of prorenin. Second, the RER induces a signal transduction cascade upon ligand binding involving activation of the MAP (mitogen-activated protein) kinases ERK1 and ERK2. Since little is known about the RER, we analyzed its function and signal transduction cascade. Initially, using RT/PCR and Northern blotting, we found evidence for an ubiquitous expression pattern of the human RER. Consistently, we were able to identify several transcriptional start sites by 5´-RACE and a high promoter activity using luciferase assays. In addition, by overexpressing tagged RER, we demonstrated an intracellular expression pattern in addition to the published cell-surface localization. RER activation by recombinant (but not extracted) renin in HeLa-S3 cells caused a strong ERK1/2 activation (+ 314% at 5 min). Interestingly, we could identify PLZF (promyelocytic zinc finger protein) as direct protein interaction partner of the RER by yeast two-hybrid screening. Our experiments indicate that the RER exhibits intracellular functions, and might - besides the activation of MAPK - transduce signals to the nucleus with PLZF as adaptor protein.