gms | German Medical Science

28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga

24. bis 27.11.2004, Hannover

The angiotensin II-receptor antagonist valsartan and the alpha-1-receptor antagonist doxazosin inhibit endothelin-1-induced vasoconstriction in the skin microcirculation

Valsartan und Doxazosin hemmen die Endothelin-vermittelte Vasokonstriktion

Meeting Abstract (Hypertonie 2004)

  • A. Mitchell - Universitätsklinikum der Universität Duisburg/Essen (Essen, D)
  • U. Ruzhentsova - Universitätsklinikum der Universität Duisburg/Essen (Essen, D)
  • J. Nürnberger - Universitätsklinikum der Universität Duisburg/Essen (Essen, D)
  • R.F. Schäfers - KfH Nierenzentrum Essen (Essen, D)
  • T. Philipp - Universitätsklinikum der Universität Duisburg/Essen (Essen, D)
  • R. Wenzel - Öffentliches Krankenhaus Zell am See (Zell am See, A)

Hypertonie 2004. 28. Wissenschaftlicher Kongress der Deutschen Hochdruckliga. Hannover, 24.-27.11.2004. Düsseldorf, Köln: German Medical Science; 2005. Doc04hochP107

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hoch2004/04hoch107.shtml

Veröffentlicht: 10. August 2005

© 2005 Mitchell et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Background: Interactions of the sympathetic nervous system (SNS), the renin-angiotensin-system (RAS) and the endothelin-system (ETS) are being implicated in the pathogenesis of cardiovascular disease. Whereas the interrelationship of SNS and RAS has been well defined, knowledge of their respective interplay with the ETS is limited. We investigated the influence of angiotensin II- (ATII) and alpha-1-receptor-antagonism on endothelin- (ET-1)-induced vasoconstriction.

Study design and methods: 20 healthy male subjects (aged 27±1 years) were included in a double blind, randomised, placebo controlled cross-over study. We used a Laser Doppler imager (moor LDI V 3.0) to evaluate changes in skin blood flow following intradermal injection of ET-1 (10-12 - 10-18 mol) after oral intake of the AT II-receptor antagonist valsartan (80 mg), the alpha-1-receptor antagonist doxazosin (4 mg), or placebo. Responses to AT II (10-12 - 10-18 mol) and noradrenaline (NA, 10-12 - 10-18 mol) were also assessed. Data were analyzed with ANOVA of the dose response curves or t-test and are expressed as arbitrary perfusion units (PU, mean ± SEM).

Results: Following placebo ET-1, AT II- and NA-injections led to a dose dependent vasoconstriction (mean max. constriction: ET-1 -115 ± 78 PU; ATII -84 ± 25 PU; NA -45 ± 35 PU). Valsartan significantly inhibited AT II- but more importantly also ET-1-induced vasoconstriction (ATII: max. +123 ± 55 PU, ET-1 max. +275 ± 144 PU, P= 0.016 and P< 0.001 vs. placebo, respectively). NA-mediated constriction was not influenced by valsartan. Doxazosin inhibited ET-1-induced constriction (max. +322 ± 156 PU, P< 0.001 vs. placebo) but did not influence constriction in response to exogenous NA and AT II.

Conclusion: Both valsartan and doxazosin inhibited ET-1-induced vasoconstriction in man in vivo, indicating a prominent role for both SNS and RAS in ET-1-mediated vascular responses. Further studies are needed to investigate the underlying mechanisms.