gms | German Medical Science

83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

16.05. - 20.05.2012, Mainz

Telomere shortening impairs regeneration of the olfactory epithelium in response to injury but not under homeostatic conditions

Meeting Abstract

  • corresponding author Masami Rudolph - Universität Ulm HNO, Ulm, Germany
  • Marc-Oliver Scheithauer - Otorhinolaryngology, University of Ulm, Ulm
  • Gerhard Rettinger - Otorhinolaryngology, University of Ulm, Ulm
  • Dietmar Rudolf Thal - Pathology, University of Ulm, Ulm
  • Karl Lenhard Rudolph Rudolph - Molecular Medicine and Max-Planck-Research Department of Stem Cell Aging, Univer, Ulm

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 83. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Mainz, 16.-20.05.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. Doc12hnod671

doi: 10.3205/12hnod671, urn:nbn:de:0183-12hnod6717

Veröffentlicht: 4. April 2012

© 2012 Rudolph et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Atrophy of the olfactory epithelium (OE) associated with impaired olfaction and dry nose represents one of the common phenotypes of human aging. Although age is the most unifying risk factor for atrophic changes and functional decline of the olfactory epithelium, little is known about molecular mechanisms that could influence maintenance and repair of the olfactory epithelium.

Methods: we analyzed the influence of telomere shortening (a basic mechanism of cellular aging) on homeostasis and regenerative reserve in response to chemical induced injury of the OE in late generation telomere knockout mice (G3 mTerc-/-) with short telomeres compared to wild type mice (mTerc+/+) with long telomeres by using immunohistochemical stainings.

Results: The study revealed no significant influence of telomere shortening on homeostatic maintenance of the OE during mouse aging. In contrast, the regenerative response to chemical induced injury of the OE was significantly impaired in G3 mTerc-/- mice compared to mTerc+/+ mice. Seven days after chemical induced damage, G3 mTerc-/- mice exhibited significantly enlarged areas of persisting atrophy compared to mTerc+/+ mice (p=0.031). Telomere dysfunction was associated with impairments in cell proliferation in the regenerating epithelium. Deletion of the cell cycle inhibitor, Cdkn1a (p21) rescued defects in OE regeneration in telomere dysfunctional mice.

Conclusions: These data indicate that telomere shortening impairs the regenerative capacity of the OE by impairing cell cycle progression in a p21-depednent manner. These findings could be relevant for the impairment in OE function in elderly people.