gms | German Medical Science

82. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

01.06. - 05.06.2011, Freiburg

Impairment of the respiratory epithelium caused by zinc oxide nanoparticle exposure?

Meeting Abstract

  • corresponding author presenting/speaker Kai Fruth - Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
  • Susanne Duca - Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
  • Anne Hilliger - Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
  • Wolf J. Mann - Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany
  • Juergen Brieger - Department of Otolaryngology, Head and Neck Surgery, University Medical Center of the Johannes Gutenberg University, Mainz, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 82nd Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Freiburg, 01.-05.06.2011. Düsseldorf: German Medical Science GMS Publishing House; 2011. Doc11hno57

DOI: 10.3205/11hno57, URN: urn:nbn:de:0183-11hno573

Veröffentlicht: 3. August 2011

© 2011 Fruth et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Background: Zinc oxide (ZnO) nanoparticles are used in industrial products like wall paint, sunscreen, and are byproducts of combustion processes. An impairment of the respiratory epithelium by ZnO-nanoparticle exposure seems feasible. However, the exact mechanism of a potential cell damage has not been investigated yet. The formation of reactive oxygen species (ROS) by ZnO nanoparticle exposure might play a significant role and influence the cell viability of the respiratory epithelium.

Methods: ROS formation and cell viability of the epithelial cell line A549 was analyzed by fluorochrome staining after exposure to ZnO-nanopaticles (size: 4–5/15–18 nm; concentration: 0.1, 10, 100 µg/ml) over different periods of time, 4, 24, 48 and 72 hrs.

Results: The impact of ZnO-nanoparticles on the reduction of cell viability and ROS formation of the respiratory epithelium was time- and dose dependent. However, differences depending on the particle size did not reach significance.

Conclusion: ZnO-nanoparticle exposure seems to influence cell viabilitiy and ROS formation and might therefore be related to respiratory cell damage and diseases of the respiratory tract.