gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

Protection of spiral ganglion cells in vivo after implantation of model electrodes coated with BDNF-producing cells

Meeting Abstract

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 81st Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hno079

DOI: 10.3205/10hno079, URN: urn:nbn:de:0183-10hno0790

Veröffentlicht: 6. Juli 2010

© 2010 Warnecke et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Long-term drug delivery to the inner ear may be achieved by functionalization of cochlear implant electrodes with cells providing neurotrophic factors to the spiral ganglion cells (SGC). In a previous study we demonstrated survival of NIH3T3/BDNF cells on model electrode surfaces, release of bioactive BDNF and protection of SGC in vitro. Aim of the present study was to investigate a potential neuroprotective effect of such modified model electrodes in vivo on deafened guinea pigs.

Lentivirally modified NIH3T3 cells producing green fluorescent protein and brain-derived neurotrophic factor (BDNF) were seeded on round silicone model electrodes (ME) at a density of 1,75x104 cells/ME and allowed to adhere and proliferate for 7 days in 48-well plates. Cell-coated model electrodes were then implanted unilaterally into the inner ear of deafened guinea pigs. The contralateral deafened but untreated ears served as controls. Animals were sacrificed 30 days after implantation, electrodes were explanted and cochleae were histologically evaluated for SGC density. A significantly increased density of SGC was observed in the treated implanted side (5.85±1.07; mean values±standard error of mean) when compared to the contralateral untreated side (4.12±1.07; p<0.01). Our data demonstrated that cell-coated model electrodes used for the local delivery of BDNF to the inner ear of guinea pigs significantly protected SGC from degeneration after ototoxic deafening.