gms | German Medical Science

81. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

12.05. - 16.05.2010, Wiesbaden

Cytogenic characterization of juvenile angiofibroma

Meeting Abstract

  • author presenting/speaker Philipp Kulas - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany
  • Silke Wemmert - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany
  • Ulrike Bechtel - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany
  • Steffi Urbschat - Klinik für Neurochirurgie, Homburg, Germany
  • Monika Hoffmann - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany
  • Vivienne Willnecker - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany
  • corresponding author Bernhard Schick - Klinik für Hals-Nasen-und Ohrenheilkunde, Homburg, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 81st Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Wiesbaden, 12.-16.05.2010. Düsseldorf: German Medical Science GMS Publishing House; 2010. Doc10hno006

DOI: 10.3205/10hno006, URN: urn:nbn:de:0183-10hno0064

Veröffentlicht: 6. Juli 2010

© 2010 Kulas et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Even juvenile angiofibromas impose histologically as benign tumors, an aggressive growth behaviour is quite frequent. Until now, only few studies focused on genetic abnormalities of these rare tumors and investigations are mainly restricted on tissue sections. Aim of the presented study was to establish cell culture lines from juvenile angiofibromas, in order to extend the options of investigating tumor biology.

Methods: Tumor tissues were cultured and the cells were characterized with the cell-type specific markers vimentin and CD31. The tumor DNA isolated from the cultured cells was analyzed by comparative genomic hybridization and the results were compared to the findings of CGH analysis of native tumor tissue.

Results: Up to now, six angiofibromas were successfully cultivated and characterized.The immunofluorescence analyses showed a purely mesenchymal cell line in all six angiofibromas. Using the CGH method, alterations found in native tissue could also be detected in two of the analyzed tumors cells after cultivation (losses on chromosomes 4 an 7, respectively, gain of chromosome 19). Furthermore, additional chromosomal alterations were observed in the cultivated tumor cells (losses on chromosome 5,9p,13p, gains of chromosomes 16,17,20,22).

Conclusion: Our preliminary results demonstrated that mesenchymal cells of juvenile angiofibromas can be cultivated successfully. Therefore, this approach could serve as basis for further analyses, which is of enormous significance considering the rarity of these tumors. As far as cell cultivation may change the representative karyotype needs to be elucidated in further examinations.