gms | German Medical Science

76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V.

04.05. - 08.05.2005, Erfurt

In Vitro Culture of Cells from Oral Mucosa on Foils of Collagen, Poly-L-Lactide (PLLA) and Poly-3-Hydroxy-Butyrate (PHB)

Meeting Abstract

Suche in Medline nach

  • corresponding author Steffen Dommerich - University of Rostock, ENT-Department, Rostock
  • Jürgen Ostwald - University of Rostock, ENT-Department, Rostock
  • Karoline Ziss - University of Rostock, ENT-Department, Rostock
  • Burkhard Kramp - University of Rostock, ENT-Department, Rostock

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 76. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e.V.. Erfurt, 04.-08.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc05hno072

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/hno2005/05hno186.shtml

Veröffentlicht: 22. September 2005

© 2005 Dommerich et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

The replacement of pharyngeal mucosa after surgical resections at different locations in the ENT-field seems to be essential or at least of great benefit for patients.

Cell cultures are initiated mainly by growing out cells from tissue pieces but also by seeding of cells after enzymatic dissociation of mucosa. Cells were cultured with serum-containing and serum-free media on different foils of collagen, Poly-L-Lactid (PLLA) and Poly-hydroxybutyric acid (PHB). Culturing time was on average about 4-5 weeks.

On all tested materials fibroblasts and epithelial cells have grown in principle. PLLA was the material with best properties concerning the aim of this study wheras culturing of cells on PHB only happens after surface modification by aminofunctionalisation with plasma treatment. The cultures were analysed by cell membrane staining and by REM.

In principle the analysed materials are useful for culturing of cells from oral mucosa. PLLA was the material with best properties. However, for obtaining differentiated epithelial cells the culture conditions have to be modified.