Artikel
Single cell gene expression profiling and assessment of therapeutic functionality of adipose-derived stem cells from the subcutaneous and visceral compartments
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Veröffentlicht: | 16. August 2017 |
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Gliederung
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Introduction: Adipose-derived stem cells (ASCs) are an appealing source of stem cells for wound healing and tissue regeneration applications due to their abundance and extensive cytokine secretion. ASCs are typically isolated from the subcutaneous compartment; however, they can also be obtained from visceral fat, and there is mixed data on the equivalency of these stem cell populations. This study analyzes the functional and single-cell microfluidic profiles of cells obtained from the subcutaneous (S-ASCs) and visceral (V-ASCs) compartments in order to better understand the therapeutic potential of these cell types.
Methods: Subcutaneous and visceral ASCs were isolated from murine fat pads and omental tissue, respectively, and applied to a “humanized” murine excisional wound model utilizing a biomimetic hydrogel scaffold. Human S- and V-ASCs were isolated from multiple patients, and their transcriptional profiles were compared across 96 genes on a single-cell level using a combined microfluidic and information theoretic approach.
Results: On a single cell level, S- and V-ASCs displayed disparate transcriptional profiles, highlighted by significant differences in the commonly utilized stem cell markers CD44 and CD105, as well as genes relating to vasculogenesis and tissue remodeling (such as Angpt1, Fgfr2, and Pdgfra). Nonetheless, application of both S- and V-ASCs equally accelerated the healing rate, time to closure and vascularity of murine excisional wounds compared to unseeded scaffold controls.
Conclusion: The significantly different transcriptional patterning of ASCs derived from the subcutaneous and visceral compartments illustrates the non-equivalency of these cell types. While their therapeutic potential is similar, the specific signaling pathways by which they act are likely unique.