gms | German Medical Science

43. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen e. V. (DGPRÄC), 17. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen e. V. (VDÄPC)

13.09. - 15.09.2012, Bremen

Optimization of Flexor Tendon Tissue Engineering: Human Adipoderived Stem Cell-Tenocyte Co-Cultures for Seeding of an Acellularized Tendon Scaffolds

Meeting Abstract

  • presenting/speaker A. Kraus - Otto-von-Guericke Universität, Plastische, Ästhetische und Handchirurgie, Magdeburg, Germany; Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States
  • C. Woon - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States
  • S. Raghavan - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States
  • H. Pham - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States
  • K. Megerle - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States
  • M.S.S. Choi - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States; Hanyang University Guri Hospital, Plastic & Reconstructive Surgery, Guri, South Korea
  • J. Chang - Stanford University Medical Center, Plastic & Reconstructive Surgery, Palo Alto, United States

Deutsche Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen. Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen. 43. Jahrestagung der Deutschen Gesellschaft der Plastischen, Rekonstruktiven und Ästhetischen Chirurgen (DGPRÄC), 17. Jahrestagung der Vereinigung der Deutschen Ästhetisch-Plastischen Chirurgen (VDÄPC). Bremen, 13.-15.09.2012. Düsseldorf: German Medical Science GMS Publishing House; 2012. DocFV70

DOI: 10.3205/12dgpraec081, URN: urn:nbn:de:0183-12dgpraec0819

Veröffentlicht: 10. September 2012

© 2012 Kraus et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Introduction: Complex hand injuries often require multiple tendon grafting. The supply of autologous grafts is limited, so that there is a demand for artificial tendon grafts. Seeding acellularized tendons with cells is an approach to provide grafts with favorable biomechanical properties. It was our aim to evaluate if human adipoderived stem cells (ASCs) could replace tenocytes for scaffold seeding.

Methods: ASCs and tenocytes were co-cultured in different ratios (3:1. 1:1, 1:3) and with three different methods (1. direct co-culture, 2. tenocyte-conditioned media on ASCs and 3. and an insert system to keep both cell types in same media without contact). Proliferation, collagen production and tenogenic marker expression were measured by hematocytometry, immunocytochemistry, ELISA and real-time PCR.

Results: Proliferation and collagen production were similar for tenocytes and ASCs alone. Tenascin C was a reliable tenocyte marker. Proliferation was increased in direct co-culture, especially at an ASC:tenocyte ratio of 3:1, and for tenocytes in ASC-conditioned media. Direct co-culture caused significant upregulation in tenascin C expression in ASCs (4.0x, p<0.05). In tenocyte-conditioned media, tenascin C expression was upregulated 2.5x (p<0.05). In the insert system, tenascin C expression was upregulated 2.3x (p<0.05).

Conclusion: ASCs are good candidates for tendon tissue engineering because they are similar to tenocytes in proliferation and collagen production. They increase proliferation in co-culture and adapt a tenocyte-like phenotype. An ASC:tenocyte ratio of 3:1 is optimal to provide good proliferation and phenotype change by minimizing donor morbidity.