gms | German Medical Science

58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

26. bis 29.04.2007, Leipzig

Endothelial and glioma cell proliferation and migration are enhanced by platelet-secreted cytokines in vitro

Migration und Proliferation von Glioblastom- und Endothelzellen werden durch plättchensezernierte Zytokine gesteigert

Meeting Abstract

Suche in Medline nach

  • corresponding author M.A. Brockmann - Universitätsklinikum Mannheim, Abteilung für Neuroradiologie, Mannheim
  • I. Nölte - Universitätsklinikum Mannheim, Abteilung für Neuroradiologie, Mannheim
  • B. Bender - Universitätsklinikum Mannheim, Abteilung für Neuroradiologie, Mannheim
  • C. Groden - Universitätsklinikum Mannheim, Abteilung für Neuroradiologie, Mannheim

Deutsche Gesellschaft für Neurochirurgie. 58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC). Leipzig, 26.-29.04.2007. Düsseldorf: German Medical Science GMS Publishing House; 2007. DocDO.02.01

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2007/07dgnc011.shtml

Veröffentlicht: 11. April 2007

© 2007 Brockmann et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective: Thrombocytosis is associated with shorter survival time in patients with glioblastoma. Platelets store different cytokines including PDGF and VEGF. Secretion of platelet storage pools during platelet activation in a potentially prothrombotic tumor microcirculation has been discussed to promote tumor growth and angiogenesis, especially in patients with thrombocytosis. This study aims to investigate platelet-tumor interaction in vitro.

Methods: Different concentrations of platelets were washed with PBS and activated. Effects of platelet secreted cytokines on endothelial and glioma cell migration were analysed using a modified Boyden chamber assay. Platelet secreted cytokines were used to trigger migration of HUVECs and glioma cell lines U87 and U373. Similarly, HUVEC and glioma cell proliferation in response to platelet secreted cytokines was analysed. HUVECS were used to perform a tube formation assay under the influence of different concentrations of platelet released cytokines.

Results: Platelet released cytokines induced strong and significant chemotactic effects in a dose dependent manner on cell lines U87 and U373 and on HUVECs. Checker board analysis revealed platelet secreted cytokines to excert mainly chemotactic, but not chemokinetic effects. Proliferation of U87 and U373 was significantly increased in response to platelet secreted cytokines in a dose dependent manner. Proliferation of HUVECs was only moderatly increased after co-incubation platelet secreted cytokines. In the in vitro angiogenesis assay capillary like tube formation and branching was significantly increased by addition of platelet secreted cytokines.

Conclusions: The results substantiate speculations, that platelets might play a role in tumor growth and angiogenesis. Additional in vivo experiments are on their way to gain further insight in platelet-tumor interaction.