gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Distribution of ezrin in interferon-gamma-induced apoptosis of primary glioblastoma cultures

Verteilung von Ezrin bei Interferon-g-induzierter Apoptosis von primären Gliomzellkulturen

Meeting Abstract

  • corresponding author W. Krupp - Dept. of Neurosurgery, University Leipzig
  • P. Stoldt - Edinger Institute, University Frankfurt
  • A. Goldammer - Dept. of Neurosurgery, University Leipzig
  • A. Derouiche - Institute of Anatomy, University Freiburg
  • J. Meixensberger - Dept. of Neurosurgery, University Leipzig
  • K. D. Geiger - Institute of Neuropathology, University Leipzig

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. DocP150

Die elektronische Version dieses Artikels ist vollständig und ist verfügbar unter: http://www.egms.de/de/meetings/dgnc2005/05dgnc0418.shtml

Veröffentlicht: 4. Mai 2005

© 2005 Krupp et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.de). Er darf vervielf&aauml;ltigt, verbreitet und &oauml;ffentlich zug&aauml;nglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.


Gliederung

Text

Objective

The actin-binding protein ezrin is related to malignancy and invasivity of glial tumors. Furthermore a link was described between ezrin expression and the induction of apoptosis in tumor cells. With the aim of finding a parameter to predict the response of glioblastoma cells to irradiation or chemotherapy, we used a paradigm of IFN-γ induced tumor cell apoptosis to study effects on the distribution of ezrin, comparing 16 primary glioblastoma cultures to the glioblastoma cell line U373MG.

Methods

Primary cell cultures were grown in tissue culture flasks and on multi chamber slides to 2/ confluency and then treated with IFN-γ (100ng/ml) versus controls with serum free medium only in at least 3 parallel essays. We established proliferation (MIB-1) and expression of ezrin (ab3C12) by immunofluorescence, and apoptosis with the TUNEL method. Ezrin distribution was quantified using laser scanning microscopic line scans in defined distances to the nucleus.

Results

All primary cell cultures showed increased induction of apoptosis following application of IFN-γ, whereas no effect was observed in the cell line. Proliferation was only marginally influenced. Ezrin-expression decreased and was altered in apoptotic cells but not in surviving cells of IFN-γ - treated cultures, which however showed increased distribution of ezrin to the nucleus.

Conclusions

These results link the distribution of ezrin to the induction of apoptosis by IFN-γ. Ezrin is redistributed and finally lost during apoptosis. Ezrin nuclear seggregation in surviving tumor cells may represent the morphological correlate of a therapy escape mechanism in glioblastoma cells.