gms | German Medical Science

56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)
3èmes journées françaises de Neurochirurgie (SFNC)

Deutsche Gesellschaft für Neurochirurgie e. V.
Société Française de Neurochirurgie

07. bis 11.05.2005, Strasbourg

Alterations of cannabinoid mediated vascular reactivity after traumatic brain injury

Veränderungen der zerebrovaskulären Reaktivität auf Cannabinoide nach fokalem und globalem Schädelhirntrauma

Meeting Abstract

Suche in Medline nach

  • corresponding author A. Menke - Neurochirurgische Universitätsklinik, Universität Freiburg
  • L. Schilling - Neurochirurgische Forschung, Fakultät für Klinische Medizin Mannheim, Universität Heidelberg

Deutsche Gesellschaft für Neurochirurgie. Société Française de Neurochirurgie. 56. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), 3èmes journées françaises de Neurochirurgie (SFNC). Strasbourg, 07.-11.05.2005. Düsseldorf, Köln: German Medical Science; 2005. Doc09.05.-13.06

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Veröffentlicht: 4. Mai 2005

© 2005 Menke et al.
Dieser Artikel ist ein Open Access-Artikel und steht unter den Creative Commons Lizenzbedingungen ( Er darf vervielfältigt, verbreitet und öffentlich zugänglich gemacht werden, vorausgesetzt dass Autor und Quelle genannt werden.




Cannabinoid ligands are supposed to exert neuroprotective mechanisms after traumatic brain injury (TBI). The mechanisms underlying this neuroprotection are not yet clear, and they may include vascular and non-vascular actions. The pursuit of the study was, therefore, to characterise cannabinoid mediated cerebrovascular relaxation following experimental focal or global cerebral trauma in male Sprague-Dawley rats.


TBI was induced in animals anaesthetised with chloral hydrate (360 mg /100 g i.p.). Focal trauma: skull bone was thinned over the right parietal cortex and a cold lesion (CL) induced by applying a cold probe onto the intact tabula interna (diameter, 4 mm; temperature, -68°C; duration, 6 min). Global trauma: weight drop (WD) on the unprotected skull (weight, 350g; height, 28.5cm). 24 or 48 h after TBI, rats were killed and ring segments of the basilar artery (BA), the middle cerebral artery (MCA), and the mesenteric artery (MA) were prepared for measurement of isometric force. Relaxation was tested in precontracted arteries (BA: 1μM serotonin; MCA: 3μM U46619; MA: 30μM UTP) by cumulative application of meth-anandamide (Meth-A, a cannabinoid-mimetic).


In MCA segments relaxation upon Meth-A was significantly enhanced, mainly with respect to the affinity after TBI. This increase was apparent in the segments taken from the proximal and the distal part. The pD2 values (mean±SD) obtained in the distal segments after CL were 5.47±0.36 (CL, 24h) and 5.36±0.24 (CL, 48h), 5.49±0.16 (WD, 24h) and 5.48±0.31 (WD, 48h). All values are significantly higher than the control value (4.99±0.17). In the BA there was no significant shift of the pD2 value after TBI, while in the MA the affinity of Meth-A was unaltered (after CL) or decreased (after WD). The maximum relaxation obtained in MCA segments was slightly increased after CL and significantly augmented after WD, unaltered after both, CL and WD trauma in the BA, and significantly attenuated in the MA after focal and global TBI.


After focal and global TBI cerebrovascular reactivity upon Meth-A was maintained or even enhanced, while it was diminished in the MA. These alterations suggest that the cannabinoid system may be a new therapeutic approach for the treatment of TBI patients. In addition, the results strongly suggest systemic alterations in vascular function to occur after head injury. The pathophysiological role of these systemic alterations needs to be further addressed.