Artikel
Comparative Protein-Profiling of Ulcerative Colitis associated Colorectal Cancer and Sporadic Colorectal Cancers
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Autoren
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Sophie Kollbeck
- University of Hamburg Medical Institutions, Dept of General Visceral and Thoracic Surgery, Hamburg, Deutschland
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Timo Gemoll
- University of Lübeck and UKSH, Campus Lübeck, Department of Surgery-Section for Translational Oncology and Biobanking, Lübeck, Deutschland
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Karl F. Karstens
- University of Hamburg Medical Institutions, Dept of General Visceral and Thoracic Surgery, Hamburg, Deutschland
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Jens K. Habermann
- University of Lübeck and UKSH, Campus Lübeck, Department of Surgery-Section for Translational Oncology and Biobanking, Lübeck, Deutschland
| Veröffentlicht: | 21. April 2016 |
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Gliederung
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Background: Patients with long-standing ulcerative colitis are at increased risk of neoplastic development. Ulcerative colitis associated colorectal carcinoma (UCC) are often difficult to detect endoscopically and to discriminate from inflammatory regenerative epithelium. Therefore a molecular marker for early stage UCC is urgently required. While changes during carcinogenesis have been thoroughly studied in SCC, minor is known about UCC. This study aimed to identify divergence from protein expression profile of sporadic colorectal carcinoma (SCC) versus UCC. Such markers might serve for better understanding, detection and therapeutic intervention of UCC.
Materials and methods: In this study, a unique series of fresh frozen UCC samples paired adjacent sporadic colon cancer tissue was investigated. Cancerous intestinal mucosal cells of patients with SCC (n=10) and patients with UCC (n=9) were obtained by Laser Capture Microdissection. Proteins of the 2 groups were isolated and then compared with multiplex-fluorescence two-dimensional gel electrophoresis (2-DE)-based protein expression pattern. 2-DE results with differences in expression levels between the two groups were analyzed with SameSpot® software followed by principal component analysis (PCA) and identification via mass spectrometry.
Results: Comparing the proteome of SCC and UCC cancer cells (p < 0.05), 199 differentially expressed proteins could be detected. Compared with sporadic colorectal cancer mucosa (SCC), protein abnormal expression of 108 spots were up-regulated an 91 were down-regulated. A mass spectometry-based analysis identified 67 of these polypeptides containing 43 unique proteins.
Conclusion: This study showed and identified significant differences in protein expression of colorectal carcinoma cells from UCC patients compared to patients with SCC. These targets have been identified by mass spectrometry. They are currently validated by Western blotting in comparison to non-cancerous intestinal mucosal cells in order to further evaluate their biomarker potential.
Figure 1 [Fig. 1]
