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21st Annual Meeting of the German Retina Society and 8th Symposium of the International Society of Ocular Trauma (ISOT)

German Retina Society
International Society of Ocular Trauma

19.06. - 22.06.2008, Würzburg

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The Effect of OECs Xenotransplantation on Post-trauma Reparation and Functional Activity of the Retina

Meeting Abstract

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  • Ekaterina Chentsova - Moscow/Russia
  • I. Kuprashvili - Moscow/Russia
  • M. Zueva - Moscow/Russia
  • I. Tsapenko - Moscow/Russia
  • I. Saburina - Moscow/Russia

Retinologische Gesellschaft. International Society of Ocular Trauma. 21. Jahrestagung der Retinologischen Gesellschaft gemeinsam mit dem 8. Symposium der International Society of Ocular Trauma. Würzburg, 19.-22.06.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. DocISOTRG2008V020

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/rg2008/08rg020.shtml

Published: June 18, 2008

© 2008 Chentsova et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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The purpose of this work was to study the regenerative and neuroprotective effects of Olfactory Ensheathing Cells (OECs) obtained from the olfactory zone of the mucous membrane of the nose, using the laser-induced rabbit retina injury model.

Methods: The retina of 10 rabbits (20 eyes) was coagulated by an argon laser. OECs were injected into the suprachoroidal space of experiment eyes; the same amount of BSS was injected in sham eyes. Elecroretinographic (ERG) and histological studies were carried out. General ERG (1HZ stimulation frequency) and 30Hz-flicker ERG were registered before and after 7-14- 30 days after injury of the retina and OECs transplantation. Histological studies were conducted: the linear size of the retina that contained no photoreceptors was measured on the 30th day.

Results: ERG results indicate that suprachoroidal injection of OECs had neuroprotective effect on photorecepor and Mueller cell functions. Compared with shams, OECs transplantation helped preserve the retinal function and later it improved the restoration of retina electrogensis.Morphometric measurements confirmed that the average size of all affected sections of the retina in experiment eyes were considerably smaller than in shams.

Conclusions: Our findings show that OECs transplantation after laser-induced injury of the retina facilitates its rapid regeneration, probably due to the presence of neural stem cells in OEC culture.