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79th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

30.04. - 04.05.2008, Bonn

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Oral squamous cell carcinoma derived stromal cells influence phenotypic characteristics of keratinocytes

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  • corresponding author Martin Chovanec - Dep. of Otorhinolaryngology and Head and Neck Surgery, Prague 5, Czech Republic
  • Karel Jr. Smetana - Institute of Anatomy, First Faculty of Medicine, Charles University, Prague 2, Czech Republic
  • Lukas Lacina - Department of Dermatovenerology, General Faculty Hospital, First Faculty of Medicine, Prague 2, Czech Republic
  • Barbora Dvorankova - Center of Cell Therapy and Tissue Repair, Second Faculty of Medicine, Charles University, Prague 5, Czech Republic
  • Zdenek Cada - Dep. of Otorhinolaryngology and Head and Neck Surgery, Prague 5, Czech Republic
  • Jan Plzak - Dep. of Otorhinolaryngology and Head and Neck Surgery, Prague 5, Czech Republic
  • Jan Betka - Dep. of Otorhinolaryngology and Head and Neck Surgery, Prague 5, Czech Republic
  • Hans-Joachim Gabius - Institute of Physiological Chemistry, Ludwig-Maximilians-University, Munich
  • Sabine Andre - Institute of Physiological Chemistry, Ludwig-Maximilians-University, Munich

Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. 79. Jahresversammlung der Deutschen Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie. Bonn, 30.04.-04.05.2008. Düsseldorf: German Medical Science GMS Publishing House; 2008. Doc08hnod162

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hnod2008/08hnod162.shtml

Published: April 22, 2008

© 2008 Chovanec et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Head and neck squamous cell cancer is appreciated as disease of epithelial cells. However the microenvironment established by stromal cells could influence phenotypic aspects of epithelial cells and may be relevant for tumor and stem cell biology. We address this issue for keratinocytes using tumor-derived stroma cells in a co-culture system.

We isolated stromal cells from human oral squamous cell carcinoma tissue and studied their effect on phenotypic characteristics of normal human interfollicular keratinocytes in vitro.

Stromal fibroblasts significantly influenced immuno- and lectin cytochemical properties of co-cultured normal keratinocytes. Expression of keratins 8 and 19, the nucleolar protein nucleostemin, parameters related to adhesion/growth-regulatory galectins and the epithelial-mesenchymal transition were altered. This biological activity of tumor-derived stromal cells, which did not require cell contact, appeared to be stable, because it was maintained during passaging of keratinocytes in the absence of cancer cells.

Tumor-derived stromal fibroblasts acquire distinct properties to shape a microenvironment conducive to altering the phenotypic characteristics of normal epithelial cells in vitro. There is a potential that tumor derived stromal cells can influence phenotypic characteristics of cells of squamous epithelia in vivo and thus participate during malignant transformation.

This study was supported by the projects MSM0021620806 and 1M0021620803, NR 9049-3 and MRTN-CT- 2005-019561).