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78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2007, Munich

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Adherence behavior of human fibroblasts to suture materials

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  • corresponding author Dagmar-Ulrike Richter - Universitätsfrauenklinik im Klinikum Südstadt, Rostock, Germany
  • Bernd Gerber - Universitätsfrauenklinik im Klinikum Südstadt, Rostock, Germany
  • Ludwig Jonas - Universität, Pathology, Rostock, Germany
  • Volker Briese - Universitätsfrauenklinik im Klinikum Südstadt, Rostock, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno096

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2007/07hno096.shtml

Published: August 8, 2007

© 2007 Richter et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Industrial research (S 736) in cooperation with the following firms: SERAG Wiessner AG, PromoCell GmbH und Bioserv GmbH

Introduction: Nowadays modern medicine without suture materials is not to be thought of.

This study shall clarify in which way the adherence of fibroblasts differs to various suture materials.

Materials/Procedure: The study includes a total number of 32 different suture materials (different firms, un-/coated, non-/absorbable filament). Furthermore, commercial cells and primary cells (subepithelial tissues) were used. For every filament beginning a concentration of 1x 100 000 cells/ml was applied. The cultivation was carried out for 10 days. The evaluation resulted from a light- and electron microscopic analysis. Simultaneously, the vitality determination was specified by trypane blue staining. For the cell characterisation the marker vimentin, and for the verification of the purity of the cell culture the marker CD 68 were utilised.

Results: On the suture materials a cell adherence could be observed. The classification of this adherence was divided into three main classes (continuously good adherence +++; incomplete adherence ++; problematic adherence +). A correlation between the adherence classification and the quantity of fibroblasts could not be observed. During the period of cultivation the cell concentration increased 10- fold. Based on this results, proliferating factors could be determined. The vitality regularly amounted to ≥98%. A classification of the filaments, according to their chemical property, permitted the percental analysis of the cell adherence.

Discussion: The in-vitro examination of the tissue compatibility of biomaterials is an accepted and valid method. Living cells react very sensitive to foreign materials. Based on our study, it was the first time that in-vitro examinations were systematically carried out on suture materials, which are used in surgery.