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78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

16.05. - 20.05.2007, Munich

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Alteration on NO-production in the cochlea of the guinea pig after intratympanic Gentamicin application

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  • corresponding author Kai Helling - ENT Department, Johannes Gutenberg-University Mainz, Mainz, Germany
  • Ulf-R. Heinrich - ENT Department, Johannes Gutenberg-University Mainz, Mainz, Germany
  • M. Sifferath - ENT Department, Johannes Gutenberg-University Mainz, Mainz, Germany
  • Jürgen Brieger - ENT Department, Johannes Gutenberg-University Mainz, Mainz, Germany
  • O. Selivanova - ENT Department, Johannes Gutenberg-University Mainz, Mainz, Germany

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. 78th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery. Munich, 16.-20.05.2007. Düsseldorf, Köln: German Medical Science; 2007. Doc07hno034

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2007/07hno034.shtml

Published: August 8, 2007

© 2007 Helling et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Introduction: Gentamicin application is an essential therapeutic option for Menière’s disease. Despite given gentamicin at intervals, a destruction of the cochlea was often observed and assumed to be the result of an increased NO-production in the inner ear. The presented study was undertaken to identify any correlation between application of gentamicin, the spatiotemporal destruction pattern and the regions of altered NO-production in the cochlea.

Materials and methods: A single dosage of Gentamicin (10 mg/kg body weight) was injected intratympanally to male guinea pigs and the acoustic evoked potentials (AEPs) were recorded before treatment and 1, 2, and 7 days after application. The organ of Corti and the lateral wall were removed from the bulla, incubated separately for six hours in cell culture medium and the degree of NO-production was determined by chemiluminescence.

Results: Gentamicin application resulted in a reduction of the hearing threshold at the second day after treatment. In the organ of Corti, the amount of NO-production remained nearly unchanged, but two days after gentamicin application an increased NO-production was found in the lateral wall.

Discussion: The correlation between the alteration in hearing threshold shift and the increased NO-production in the lateral wall favors the idea that gentamicin might influence potassium recycling processes and thus contributes to the disturbance of the cochlear ion homeostasis.