gms | German Medical Science

77th Annual Meeting of the German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

German Society of Oto-Rhino-Laryngology, Head and Neck Surgery

24.05. - 28.05.2006, Mannheim

Article

Send article

Chondrocytes on polyethylene (Medpor®) - Vitality and expression of ECM molecules

Meeting Abstract

Search Medline for

  • author presenting/speaker Christian Egger - Universitäts-HNO-Klinik der MLU Halle-Wittenberg, Halle (Saale), Germany
  • corresponding author Ilona Schön - Universitäts-HNO-Klinik der MLU Halle-Wittenberg, Halle (Saale), Germany
  • Ernst Röpke - Universitäts-HNO-Klinik der MLU Halle-Wittenberg, Halle (Saale), Germany
  • Marc Bloching - Universitäts-HNO-Klinik der MLU Halle-Wittenberg, Halle (Saale), Germany

German Society of Otorhinolaryngology, Head and Neck Surgery. 77th Annual Meeting of the German Society of Otorhinolaryngology, Head and Neck Surgery. Mannheim, 24.-28.05.2006. Düsseldorf, Köln: German Medical Science; 2006. Doc06hno082

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/hno2006/06hno082.shtml

Published: September 7, 2006

© 2006 Egger et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

To optimise the conditions for cartilage tissue engineering in the laboratory the influence of different factors on the vitality of primary chondrocytes was tested. Former experiments had shown that coating polyethylene surfaces with extracellular matrix molecules facilitates chondrocyte adhesion.

Taking this background into consideration systematical tests were to be carried out to establish to what extent individual origin, number of passages, incubation times and media additives affect the vitality of chondrocytes on PE surfaces.

To determine the vitality of adherent cells results were obtained via MTT or WST tests and compared with the results of the determination of vital cells by trypan blue method.

The expression of extracellular matrix molecules was examined via immunohistochemical analysis and compared with their expression in native septal cartilage.

Outline

References

1.
Schön I, Röpke E, Pudszuhn A, Pietz O, Vogel J, Berghaus A. Optimierung von Chondrozytenkulturen auf Polyethylenoberflächen durch den Einsatz von Extrazellulärmatrixproteinen. Posterabstract, HNO Informationen 2 (2003), 188
2.
Schön I, Röpke E, Markwart A, Egger C, Bloching M. Verbesserung der Biokompatibilität von nicht resorbierbarem Knorpelimplantatmaterial durch Beschichtung mit Molekülen der Extrazellulärmatrix. Biomaterialien 6(S1), 2005, 46-47