gms | German Medical Science

104th DOG Annual Meeting

21. - 24.09.2006, Berlin

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An experimental study on Chitosan as vitreoretinal tamponde

Meeting Abstract

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  • H. Yang - Department of Ophthalmology, Tongji Hospital, Tongji Medical College of Science and Technology Unibersity, Wuhan
  • R. Wang - Department of Ophthalmology, Tongji Hospital, Tongji Medical College of Science and Technology Unibersity, Wuhan
  • Y. Xiang - Department of Ophthalmology, Tongji Hospital, Tongji Medical College of Science and Technology Unibersity, Wuhan
  • X. Zhang - Department of Ophthalmology, Tongji Hospital, Tongji Medical College of Science and Technology Unibersity, Wuhan

Deutsche Ophthalmologische Gesellschaft e.V.. 104. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft (DOG). Berlin, 21.-24.09.2006. Düsseldorf, Köln: German Medical Science; 2006. Doc06dogP169

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2006/06dog691.shtml

Published: September 18, 2006

© 2006 Yang et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

To study the effect of Chitosan to intraocular structure such as retina, ciliary body, lens,cornea etc and intraocular pressure; and to messure the levels of inflammatory factors in aqueous humor and vitreous humor following vitrectomy using Chitosan as a vitreoretinal tamponde.

Methods

Twelve healthy pigmented rabbits were vitrectomized. Then the vitreous cavities were filled by injecting Chitosan into the right eyes (as experiment group) and Hyalumnan into the left eyes (as control group). Slit-lamp biomicroscopy and indirect ophthalmoscopy were performed and intraocular pressure were messured pre- and postoperatively in all eyes. Histological and ultrapathological examination was done after the rabbits were sacrificed at the end of the study. The inflammatory factors (interleukin 6, interleukin 8, NO) levels in aqueous humor and vitreous humor were respectively detected on the 15th and 30th day afterwards.

Results

All the animal clinic examination and histological and ultrapathological examination of ocular tissues indicated that there were no significant differences between experiment group and control group. The IL-6 levels in aqueous humor of experimental group and control group on the 15th day postoperatively were higher than normal, as for the IL-8 and NO levels there were no differences when compared to normal. The IL-8 and NO levels in vitreous humor of experimental group and control group on the 30th day postoperatively were higher than normal, as for the IL-6 levels there were no differences when compared to normal. The IL-6 and IL-8 levels in aqueous humor and vitreous humor had no significant differences between experiment group and control group at all times.

Conclusions

There is no obvious effect to intraocular structure and intraocular pressure fluctuation when the vitreous cavity is filled with Chitosan. Although there is some fluctuation of inflammatory factors but no serious inflammation appears.