gms | German Medical Science

104th DOG Annual Meeting

21. - 24.09.2006, Berlin

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Rabbit’s intraocular pressure after instillation of Timolol and amino acid lysine, arginine, glycine or taurine mixture

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  • Z. Oláh - Department of Ophthalmology, Fac. of Med., Comenius University, Bratislava, Slovak Republic
  • J. Veselovský - Institute of Animal Physiol. & Ethology, Fac. of Nat. Sci., Comenius University, Bratislava, Slovak Republic

Deutsche Ophthalmologische Gesellschaft e.V.. 104. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft (DOG). Berlin, 21.-24.09.2006. Düsseldorf, Köln: German Medical Science; 2006. Doc06dogSA.11.12

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2006/06dog367.shtml

Published: September 18, 2006

© 2006 Oláh et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

The presented experimental work is focused to examine the pharmacokinetic effectivity the instillation of the mixtures of 0.5% Timolol with 4 free amino acid, present in conjunctival sac: lysine, arginine, glycine and taurine on physiological values of the IOP in rabbits.

Methods

The experimental work was performed on 5 female rabbits of the New Zealand White species. After instillation at 8oo a/ the 10% L-lysine.2HCl.2H2O in 0.5% Timolol; b/ the 10% L-arginine.HCl in 0.5% Timolol; c/ the 10% L-glycine.HCl in 0.5% Timolol; d/ the 10% L-taurine.HCl in 0.5% Timolol, and both the 0.5% Timolol and amino acids alone into the left conjunctival sac, The IOP was measured before and in 5th, 15th, 30th, 60th, 120th, 180th and 240th min. and 24 hours after the instillation. The right eye was used as control.

Results

a. The decrease of IOP after mixture of 10% lysine in 0.5% Timolol had two spikes – nonsignificant decrease up to 60th min. and high significant decrease was observed between 120th to 240th min.;

b. The mixture of 10% arginine in 0.5%Timolol high significantly decrease the IOP values (from 2.1 to 4 torr);

c. The 10% glycine in 0.5% Timolol showed significant IOP decrease without 5th min. in all measured times, the major decrease of the IOP was measured in 60th min. (mean value 8.5 torr), also in 24th hours the decrease was to 3.3 torr;

d. The 10% taurine in 0.5% Timolol showed also significant IOP decrease without 5th min. in all measured times with maximum in 180th min. (with value of decrease 6.7 torr) and in 24th hours the decrease showed still significantly lower level – 3.3 torr.

Conclusions

The results proved that in the mixture of antiglaucomatic 0.5% Timolol and amino acid (lysine, arginine, glycine or taurine) a new biologically active sub-stance, the „specific bioantiglaucomatic“ is created by interaction. Compared with the substances alone, mixture of the amino acid in the antiglaucomatic decreased the physiologic IOP in rabbits with high significance. The showed effect on IOP based on interaction of the mixture amino acid with antiglaucomatic is specific and efficacy together with duration changed according the type of amino acid. Our in vitro produced bioantiglaucomaticum fulfilled the physiological conditions for the the IOP reduction.