gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

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Epidemic keratoconjunctivitis: detecting adenovirus with the assay SAS™ Adeno-Test

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  • corresponding author J. Mielke - Universitäts-Augenklinik Tübingen und Institut für Medizinische Virologie des Universitätsklinikums Tübingen
  • N. Freudenthaler - Universitäts-Augenklinik Tübingen und Institut für Medizinische Virologie des Universitätsklinikums Tübingen
  • C.M.E. Deuter - Universitäts-Augenklinik Tübingen und Institut für Medizinische Virologie des Universitätsklinikums Tübingen
  • R. Beck - Universitäts-Augenklinik Tübingen und Institut für Medizinische Virologie des Universitätsklinikums Tübingen
  • M. Zierhut - Universitäts-Augenklinik Tübingen und Institut für Medizinische Virologie des Universitätsklinikums Tübingen

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogFR.15.04

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog294.shtml

Published: September 22, 2004

© 2004 Mielke et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

It is often difficult clinically to differentiate epidemic keratoconjunctivitis from the diagnosis "red eye". To prevent the spread of this highly contagious infection and to initiate adequate treatment an early and reliable diagnose would be very welcome. Validity of the rapid adenovirus assay (SAS™ Adeno-Test) was evaluated.

Methods

75 patients with assumed epidemic keratokonjunctivitis were included. In all patients polymerase chain reaction (PCR) of conjunctival swabs was performed to detect adenovirus. In all patients direct virus detection was carried out with the immunochromatographic assay. The case records of all patients included sex, age and beginning of the clinical symptoms. Sensitivity and specifity of the test was calculated.

Results

In 25 patients adenovirus was detected by PCR. 18/25 patients were detected by the SAS™ Adeno-Test, 7/25 were not detected by SAS™ Adeno-Test. In 50 patients there was neither virus detection by PCR nor by the SAS™ Adeno-Test. Sensitivity was 72%, specifity was 100%. Adenovirus was detected by SAS™ Adeno-Test in 82% of patients with clinical symptoms less than 5 days, but only in 50% of patients with clinical symptoms of 5 and more days.

Conclusions

The results indicate that rapid adenovirus detection with the SAS™ Adeno-Test is a useful tool in patients with assumed epidemic keratokonjunctivitis and clinical symptoms of less than 5 days. In patients with clinical symptoms of 5 days or more virus detection should be performed by PCR additionally.