gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

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Photodynamic-therapy-induced apoptosis in choroidal neovascularization membranes

Meeting Abstract

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  • corresponding author K. Petermeier - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • O. Tatar - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • F. Gelisken - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • P. Szurman - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • M. Völker - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • K. U. Bartz-Schmidt - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen
  • S. Grisanti - Eberhard-Karls-Universität Tübingen, Universitäts-Augenklinik, Abt. I, Tübingen

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogFR.03.08

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog184.shtml

Published: September 22, 2004

© 2004 Petermeier et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

Text

Objective

Choroidal neovascularization membranes (CNVM) three days after Verteporfin photodynamic therapy (PDT) disclose both occluded as well as patent vessels. At later time points after PDT, however, all vessels are patent and lined by healthy endothelial cells. The present study was performed to distinguish whether these vessels originate from the treated ones or result from neoangiogenesis.

Methods

12 surgically excised CNVM following treatment with PDT were analyzed. Apoptosis was detected by staining with the TUNEL technique and compared with the expression of the panendothelial marker CD 34, CD 105, a marker for activated endothelial cells and the nuclear cell proliferation marker Ki 67.

Results

In CNVM 3 days after PDT, the endothelial cells even of the patent appearing vessels were apoptotic. The endothelial cells in CNVM 1 - 5 months after PDT showed no TUNEL-positive reaction, but a distinct expression of markers for cell proliferation.

Conclusions

PDT induces apoptosis in endothelial cells. The regeneration of apoptotic cells is improbable. Therefore, endothelial cells lining patent vessels in CNVM excised 1 - 5 month after PDT are the result of neoangiogenesis rather than recanalization.