gms | German Medical Science

102. Jahrestagung der DOG

Deutsche Ophthalmologische Gesellschaft e. V.

23. bis 26.09.2004, Berlin

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Intraocular growth factors are not altered in early stages of diabetes mellitus

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  • corresponding author K. Höhne - Deutsches Institut für Ernährungsforschung, Abteilung für klinische Ernährung, Potsdam-Rehbrücke, und Charité-Universitätsmedizin, Campus Benjamin Franklin, Abteilung für Endokrinologie, Diabetes und Ernährungsmedizin; , Charité-Universitätsmedizin, Campus Benjamin Franklin, Abteilung für Endokrinologie, Diabetes und Ernährungsmedizin; Charité-Universitätsmedizin, Campus Benjamin Franklin, Abteilung für Endokrinologie, Diabetes und Ernährungsmedizin; Charité-Universitätsmedizin, Campus Benjamin Franklin, Klinik und Poliklinik für Augenheilkunde, Potsdam
  • H. Heimann - Charité-Universitätsmedizin, Campus Benjamin Franklin, Klinik und Poliklinik für Augenheilkunde, Potsdam
  • J. Spranger - Deutsches Institut für Ernährungsforschung, Abteilung für klinische Ernährung, Potsdam-Rehbrücke, und Charité-Universitätsmedizin, Campus Benjamin Franklin, Abteilung für Endokrinologie, Diabetes und Ernährungsmedizin, Potsdam
  • N. E. Bechrakis - Charité-Universitätsmedizin, Campus Benjamin Franklin, Klinik und Poliklinik für Augenheilkunde, Potsdam
  • A. F. H. Pfeiffer - Deutsches Institut für Ernährungsforschung, Abteilung für klinische Ernährung, Potsdam-Rehbrücke, und Charité-Universitätsmedizin, Campus Benjamin Franklin, Abteilung für Endokrinologie, Diabetes und Ernährungsmedizin, Potsdam
  • M. H. Foerster - Charité-Universitätsmedizin, Campus Benjamin Franklin, Klinik und Poliklinik für Augenheilkunde, Potsdam

Evidenzbasierte Medizin - Anspruch und Wirklichkeit. 102. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft. Berlin, 23.-26.09.2004. Düsseldorf, Köln: German Medical Science; 2004. Doc04dogDO.03.10

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dog2004/04dog027.shtml

Published: September 22, 2004

© 2004 Höhne et al.
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Outline

Text

Objective

Proliferative diabetic retinopathy is characterized by an imbalance of angiogenic and angioinhibitory intraocular cytokines. Thus we investigated PEDF and VEGF levels in early stages of diabetes mellitus with no clinical signs of retinal damage.

Methods

We obtained vitreous of 16 diabetic patients and 31 nondiabetic patients undergoing routine vitrectomy due to macular hole, epiretinal gliosis or rhegmatogenous retinal detachment. None of these patients showed any signs of diabetic retinopathy. Albumin levels were measured by standard techniques. PEDF vitreous levels were determined by western blot analysis. VEGF was measured with ELISA. PEDF and albumin are provided as means±SEM, VEGF levels are provided as medians.

Results

Albumin levels are not different in diabetics and controls (0,32 g/l±0,07 g/l versus 0,34 g/l±0,07 g/l, p=0,8). PEDF levels differ in the three disease groups but not in diabetics and non-diabetics (3,6 μg/ml±0,3 μg/ml and 3,32 μg/ml±0,29 μg/ml; p=0,5, respectively) within one group. VEGF is detectable in 7 samples of controls (median 6,3 pg/ml) and in 2 samples of diabetics (median 6,2 pg/ml). This difference is not significant.

Conclusions

There is no difference in albumin levels and levels of the cytokines PEDF and VEGF in nondiabetics and diabetics without diabetic retinopathy. We conclude that first hyperglycemia induces local hypoxia through various pathways which then leads to an increase in angiogenic cytokines as PEDF and VEGF with the development of proliferative diabetic retinopathy.