gms | German Medical Science

27th German Cancer Congress Berlin 2006

German Cancer Society (Frankfurt/M.)

22. - 26.03.2006, Berlin

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Identification of genes, biological processes and pathways involved in the chromosome 6 mediated suppression of tumorigenicity in MDA-MB-231 breast cancer cells

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  • corresponding author presenting/speaker Jana Strissel - Max Delbrueck Center for Molecular Medicine, Berlin, Deutschland
  • Susanne Seitz - Max Delbrueck Center for Molecular Medicine, Berlin
  • Wolfgang Arnold - atugen AG, Berlin
  • Jörg Weimer - University Hospital Schleswig-Holstein Campus Kiel, Kiel
  • Norbert Arnold - University Hospital Schleswig-Holstein Campus Kiel, Kiel
  • Siegfried Scherneck - Max Delbrueck Center for Molecular Medicine, Berlin

27. Deutscher Krebskongress. Berlin, 22.-26.03.2006. Düsseldorf, Köln: German Medical Science; 2006. DocPE097

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dkk2006/06dkk207.shtml

Published: March 20, 2006

© 2006 Strissel et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Background: Several cytogenetic studies demonstrated frequent allelic losses at defined regions on chromosome 6 in breast tumors, suggesting the presence of tumor suppressor genes (TSGs) contributing to breast cancer tumorigenesis. Different techniques identified several candidate TSGs on chromosome 6 in breast cancer, but no functional evidence for a TSG function for these genes could be supplied so far.

Method: In order to identify key genes and elucidate the regulatory pathways that are involved in the development and progression of breast cancer, we combined array-based expression profiling with the microcell mediated transfer of chromosome 6 into MDA-MB-231 breast cancer cells.

Results: Microcell mediated transfer of chromosome 6 into MDA-MB-231 breast cancer cells resulted in hybrids characterised by reduced anchorage dependent growth and a strongly reduced tumorigenicity in nude mice. Comparing the expression profiles of tumorigenic parental cells and non-tumorigenic hybrids we identified a defined set of genes involved in chromosome 6 mediated breast cancer suppression. These genes could be integrated into biological processes and pathways known to be involved in breast cancer development. Overexpression and knock down of single genes of the identified gene set in breast and other cancer cell lines are used to further elucidate their role in cancer development and / or progression.

Conclusions: Our results provide functional evidence, that the suppression of the tumorigenic phenotype of the breast cancer cell line MDA-MB-231 is mediated by a set of genes / pathways regulated by one or more genes on chromosome 6.