gms | German Medical Science

58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e. V. (DGNC)

Deutsche Gesellschaft für Neurochirurgie (DGNC) e. V.

26. bis 29.04.2007, Leipzig

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Interactions of neural stem cells and glioma cells in vitro. Do neural stem cells augment glioma cell migration?

Interaktionen zwischen neuralen Stammzellen und Gliomzellen. Wird durch neurale Stammzellen eine Migration von Gliomzellen verstärkt?

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  • corresponding author D. Langhans - Klinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf
  • D. Zirkel - Klinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf
  • M. Westphal - Klinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf
  • K. Lamszus - Klinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf
  • O. Heese - Klinik für Neurochirurgie, Universitätsklinikum Hamburg-Eppendorf

Deutsche Gesellschaft für Neurochirurgie. 58. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC). Leipzig, 26.-29.04.2007. Düsseldorf: German Medical Science GMS Publishing House; 2007. DocP 078

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2007/07dgnc333.shtml

Published: April 11, 2007

© 2007 Langhans et al.
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Objective: Various in vivo studies have demonstrated a migration tendency of neural stem cells (NSCs) towards intracranial gliomas, making these cells a potential carrier for delivery of therapeutic genes to disseminated gliomas. Little is known about the direct effects of NSC on glioma biology. We analyzed glioma and NSC migration in response to conditioned media of the corresponding cell type in different in vitro assays.

Methods: 5 glioma cell lines were exposed to conditioned media of the murine neural stem cell line C17.2 and cell migration (5 days) was assessed. With the identical experimental set up C17.2 neurospheres were exposed to conditioned media of the 5 glioma cell lines. In addition in a organotypic brain slice assey the effects of either conditioned media of glioma cells on NSC migration and the effects of NSC conditioned media on glioma migration was evaluated using a confocal laser microscope on day 2, 6 and 12. NSCs and glioma cells were identified inside the standardized murine brain slice by pre-implantation staining with DiI and DiO.

Results: In 3 of 5 glioma cell lines migration and invasion was augmented by NSC conditioned media. No inhibitory effect of NSC conditioned media on glioma migration was seen at all. Vice versa conditioned media of glioma cells augmented NSC migration very heterogeneously ranging from almost no stimulation in 2 glioma cell lines to strong stimulation in 1 glioma cell line. Co-culturing of NSCs and glioma cells inside the brain slice resulted in a directed migration of both cell types towards each other in 3 of 5 glioma cell lines.

Conclusions: We demonstrated in 2 different in vitro assays a stimulatory effect of NSC conditioned media on glioma cell migration and invasion making the postulated hypothesis of an intrinsic glioma-inhibitory effect of NSC questionable. On the other hand migration of NSCs towards gliomas in our assay system seems to depend on individual phenotypic characteristics and growth factor release patterns of the target glioma tissue.