gms | German Medical Science

57th Annual Meeting of the German Society of Neurosurgery
Joint Meeting with the Japanese Neurosurgical Society

German Society of Neurosurgery (DGNC)

11 - 14 May, Essen

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Effect of transplantation mode on migration, differentiation and longterm survival of transplanted neural stem cells and their clinical benefit after experimental traumatic brain injury in the rat

Auswirkungen des Transplantationsverfahrens auf das Migrations- und Differenzierungsverhalten von transplantierten neuralen Stammzellen und auf das neurologische Outcome nach experimentellen Schädelhirntrauma in der Ratte

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  • corresponding author M. Skardelly - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • S. Burdack - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • K. Gaber - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • F. Scheidt - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • M.U. Schuhmann - Klinik für Neurochirurgie, Universitätsklinikum Leipzig
  • J. Meixensberger - Klinik für Neurochirurgie, Universitätsklinikum Leipzig

Deutsche Gesellschaft für Neurochirurgie. Japanische Gesellschaft für Neurochirurgie. 57. Jahrestagung der Deutschen Gesellschaft für Neurochirurgie e.V. (DGNC), Joint Meeting mit der Japanischen Gesellschaft für Neurochirurgie. Essen, 11.-14.05.2006. Düsseldorf, Köln: German Medical Science; 2006. DocSO.04.01

The electronic version of this article is the complete one and can be found online at: http://www.egms.de/en/meetings/dgnc2006/06dgnc182.shtml

Published: May 8, 2006

© 2006 Skardelly et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en). You are free: to Share – to copy, distribute and transmit the work, provided the original author and source are credited.

Outline

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Objective: Currently promising therapies to repair neuronal loss after severe traumatic brain injury (TBI) and to improve neurological recovery are missing. Since TBI is inevitably associated with loss of neural tissue, it seems logic to employ therapies which replace lost tissue in an adequate organotypic way. Due to limited capacity for self repair, transplantation of multipotent stem cells (SC) seems to be a promising approach. This project aims to determine the effect of local versus systemic transplantation of human neural SCs on migration, differentiation and long-term survival of the graft and the clinical benefit after TBI in the rat.

Methods: 60 male Sprague Dawley Rats were anesthetized and subjected to controlled cortical impact (CCI) brain injury. 24h postinjury, animals received either no therapy, or an intracerebral stereotactic (~1x105 cells) or intravenous (~5x105 cells) injection of human neural forebrain stem cells. Evaluation of neurological function by the Rotarod Test and sacrifice after either 3d, 7d, 28d or 12 weeks postinjury were performed. Serial coronal brain sections were stained with a panel of antibodies to show survival, migration and differentiation.

Results: The Rotarod Test showed no significant difference between the three groups before CCI. 49d after trauma, the statistical analysis showed a significant benefit for systemic Tx in comparison with the control group (p=0,0029) and the local Tx (p=0,002). The local Tx showed a tendential but not significant benefit in comparison with the control group (p=0,09). Brain injured animals, who received either intracerebral stereotactic or intravenous injection of TAMRA stained neural stem cells (NSC), showed NSC migration into the penumbra zone of injury and survival of the graft.

Conclusions: Preliminary results suggest effective migration into the penumbra and survival of the graft after local and systemic transplantation of stem cells after TBI. Although histological studies concerning long-time survival and differentiation are ongoing, the Rotarod Test showed a significant improvement in neurological outcome.